A novel saliva-based microRNA biomarker panel to detect head and neck cancers

Abstract:

Methods: Five differentially expressed miRNAs were selected from miScript™ miRNA microarray data generated using saliva from five HNSCC patients and five healthy controls. Their differential expression was subsequently confirmed by RT-qPCR using saliva samples from healthy controls (n∈=∈56) and HNSCC patients (n∈=∈56). These samples were divided into two different cohorts, i.e., a first confirmatory cohort (n∈=∈21) and a second independent validation cohort (n∈=∈35), to narrow down the miRNA diagnostic panel to three miRNAs: miR-9, miR-134 and miR-191. This diagnostic panel was independently validated using HNSCC miRNA expression data from The Cancer Genome Atlas (TCGA), encompassing 334 tumours and 39 adjacent normal tissues. Receiver operating characteristic (ROC) curve analysis was performed to assess the diagnostic capacity of the panel.

Año de publicación:

2014

Keywords:

• The cancer genome atlas
• Non-invasive diagnostics
• miRNA expression
• Oral cancers

Fuente:

scopus