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A pH shift-based procedure to screen fructoligosaccharides fermenting yeast or bacterial strains

Abstract:

Symbiotic, a combination of short chains-carbohydrates with prebiotic properties e.g. fructooligosaccharides (FOS) and biotherapeutical microorganisms, provides positive health effects and well-being in humans and animals. However; the lack of accurate screening methods hampers the possibility to get new fructo-oligosaccharides (FOS)-fermenting yeast or bacterial strains to design potential symbiotic combinations useful for health and food industries. A valuable screening procedure to visually evaluate pure cultures of bacterial and yeast strains able to ferment FOS in liquid or solid rich media supplemented with a pH indicator is described. Using this procedure, 15 FOS-consuming strains isolated from different sources were successfully assayed using this method. This screening procedure is a useful tool in rapid large-scale detection of potential FOS fermenting-strains.