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Bothrops colombiensis venom effect on platelet aggregattion

Abstract:

The venom total of Bothrops colombiensis (VBC) on platelet aggregation in humans was studied. One hundred twenty blood samples from volunteer donors were collected in sodium citrate 3.8%. Platelet aggregation was made in platelet-rich plasma (PRP), platelets washed with buffer tyrode pH 7.35 (PLBT) and in platelet incubated with inhibitors of platelet aggregation (PIAP): acetyl salicylic acid (ASA), ticlopidine and clopidogrel; the final concentration of platelet was 250 × 10 9/L. Inducing agents used to aggregation were Epinephirine: 1×10-5M (final concentration: 11 μg/mL), Adenosin 5 Disfosphate (ADP): 7.5×10 -6M (final concentration: 2.14 μg/mL), Collagen: 1.2×10 -2 M (final concentration: 3 μg/mL), Ristocetin: 5.5×10 -4M (final concentration: 1.2 μg/mL). Final concentration of VBC (2.2×s 10 -4) was incubated with PRP, PL, PIAP to observe the platelet aggregation. Furthermore, it was also examined the effect of PRP on VBC incubed with enzymatic inhibitors such as phenyl methyl sulfonyl fluoride (PMSF) (10.20 μg/mL) and 2-Mercaptoethanol (4.57 μg/mL). As well, etilendiaminotetracetic acid (EDTA) (64.00μg/mL) was used on platelet aggregation. The total VBC produced platelet aggregation more than 80% in PRP and PLBT (P<0.0001 and P<0.01, respectively), and reverted the inhibitor effect observed in PIAP (ASA and Clopidogrel P<0.05; ticlopidine P<0.01) and it inhibited the platelet aggregation significantly on incubated platelets with PSMF (P<0.0001). It is concluded that total VBC is a potent inducer of platelet aggregation whose action is different from the ADP-dependant cyclooxigenase. Studies are required more detailed in order to identify the component (s) responsible of the platelet effect and its mechanism of action.