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COMPILATION OF Orchidaceae FAMILY SPECIES GERMPLASM IN SANTIAGO DE MENDEZ, MORONA SANTIAGO, ECUADOR

Abstract:

Anthropogenic threats to the habitat of orchids have prompted conservation initiatives such as the creation of an orchid seed germplasm bank, a key biotechnological tool for the conservation of this threatened species. The present study was divided in two stages: a field study and a lab analysis. Orchids were collected from three areas of the Santiago de Mendez, Morona Santiago region: Copal, Tres Ranchos and La Delicia; an area of 600 m(2) where 50 orchids of 15 different genus were collected and preserved ex situ in a greenhouse in the city of Macas until the capsules matured. The seeds were later removed and dried in the CIVABI lab of the Universidad Politenica Salesiana in Quito, where 12 species were stored in vacutainer tubes using the following methods: a) refrigerated in the dark at 4 degrees C, and b) under room temperature 20 +/- 2 degrees C, in light conditions. By using the 1% tetrazolium …