A sandwich-type enzyme-linked immunosorbent assay for the analysis of recombinant human interferon α-2b

Abstract:

The ability of a previously developed sandwich-type enzyme-linked immunosorbent assay (ELISA) for discriminating incorrectly folded recombinant human interferon α-2b (IFN-α2b) molecular species from multi disulphide-bonded species was investigated. This ELISA was applied to evaluate and improve the effectiveness of the renaturation of IFN-α2b, a step that is currently used in the large-scale production of IFN-α2b produced in recombinant Escherichia coli strains.

Año de publicación:

1999

Keywords:

• Elisa
• ESCHERICHIA coli
• IFN-alpha
• monoclonal antibodies
• folding

Fuente:

scopus