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Caracterización Físico - Química y determinación de actividad biológica del aceite esencial de las hojas de Renealmina thyrsoidea Subespecie thyrsoidea

Abstract:

This study aimed to characterize the chemical and physical components of the essential oil from the leaves of Renealmia subspecies thyrsoidea, besides determining its antibacterial and antifungal activity to promote a precedent for the future realization of products from this plant. The essential oil of Renealmia thyrsoidea subspecies thyrsoidea was obtained by steam stripping. The physical properties were determined; refractive indices (1,481 g) and acid indices (22,581 g). The density value was 0,8685g. The chemical composition of OE was determined by gas chromatography coupled to mass spectrometry (GC/MS) and nuclear magnetic resonance 1H (NMR1H). There were 49 compounds identified wich representing 95,33% of the oil. The most representative compounds were sylvestrene (28,78%), camphene (8,53%), β-phellandrene (6,46%), z-neoridol (6,37%), aristolochene (4,34%), adomandreno (3,69%), ɤ-copaene (3,13%). The antioxidant activity was evaluated by two spectrophotometric methods: test DPPH and ABTS assay. The IC50 values obtained were 20,86 mg/mL for DPPH and 1,33 mg/mL for ABTS. Antimicrobial activity revealed better inhibition results than strains of Gram (-) Escherichia coli ATCC8739TM and Pseudomona aeruginosa ATCC9027TM a MIC of 0,347 mg/ml for both. Facing the yeast Candida albicans ATCC10231 was 1,389 mg/ml and Candida tropicalis ATCC13803 0,347 mg/ml. The characterization of the chemical composition and determining the biological activity of the essential oil leaves Renealmia thyrsoidea subspecies thyrsoidea showed similar data against preliminary investigations with the same it.