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A simple sample treatment for the determination of enrofloxacin and ciprofloxacin in raw goat milk

Abstract:

A solid-phase extraction (SPE) procedure requiring no prior protein precipitation for the determination of enrofloxacin (ENR) and its metabolite ciprofloxacin (CIP) in goat milk with fluorescence detection (FD) was developed. Octadecyl (C18), ethyl (C2), cyclohexyl (CH) and phenyl (PH) sorbents were initially tested for this purpose. The influence of the loading variables affecting the extraction efficiency (viz. breakthrough volume and eluent composition/volume) was examined. Using C18 cartridges in combination with 5 mL of H2O (containing 2% TFA)/acetonitrile (ACN)/methanol (MeOH)/(77:15:8, v/v/v) as eluent was found to provide the best recoveries and highest efficiency. This sample preparation method provided absolutely clean extracts with recoveries up to 99.7% for ENR and 95.9% for CIP. The overall chromatographic running time was 6 min, and the retention times for ENR and CIP were 5.32 and 4.25 min …