Caracterización molecular de Basidiomycetes a partir de aislamientos y fructificaciones colectados en la Reserva Biológica San Francisco RBSF

Abstract:

Ecuador is one of the most biodiverse countries where tropical montane forests as the Biological Reserve of San Francisco (RBSF) are highly conspicuous by its singularity and rarity, with fragile ecosystems. Biodiversity is poorly recorded in areas like RBSF, especially regarding Basidiomycetes fungal diversity. Basidiomata were sampled during five months in the tropical montane forest RBSF. We attempt to isolate fungi from fresh basidiomata in solid culture media and keep dry specimens in the fungario-HUTPL. Molecular sequences from basidiomata and pure cultures were amplified for the genes ITS-5.8S and partial LSU (D1/D2). Basidiomata (186 on total) were collected, however only from few of then attempt to isolate were successful (18 for basidiomycetes and 37 for ascomycetes). Phylogenetic analysis showed high molecular diversity mainly into four orders (Agaricales; Boletales; Geastrales and Polyporales) that includes 13 families from basidiomata; two orders (Agaricales and Polyporales) that include three families from pure cultures of basidiomycetes, and three orders (Diaporthales; Hypocreales and Xylariales) that include six families from pure cultures of Ascomycetes. The most common and abundant orders correspond to Agaricales and Hypocreales respectively

Año de publicación:

2015

Keywords:

• Bosques montanos tropicales
• Análisis filogenético
• Bioquímico farmacéutico – Tesis y disertaciones académicas
• Hongos basidiomycetes

Fuente:

rraae