Caracterización molecular de hongos micorrízicos aislados a partir de cuatro especies de orquídeas epífitas, en dos pisos altitudinales de bosque montano.

Abstract:

Most plant species form symbiotic associations with fungi called mycorrhizae that facilitates the exchange of nutrients and enhances the relationship with the environment, particularly with soil. In orchids this partnership plays a key role in seed germination and seedling setting. This study focused on molecularly characterization of the fungal community isolated from four species of orchids (Cyrtochilum myanthum, Cyrtochilum flexuosum, Epidendrum hemiscleria and Epidendrum dalessandroi) in two populations of montane forest in southern Ecuador. Roots of 10 individuals of each species were collected to attempt fungal isolate in culture media. DNA was obtained from pure cultures to amplify the ITS1-5.8S-ITS2 rDNA region using the primer combination ITS1-TW14. Sixteen of the 35 fungal isolates were identified by molecular methods. The molecular identity of the isolates was mostly members of Agaricomicetes (Basidiomycota), with isolates identified as Tulasnella. In addition, several groups of Ascomycetes as Helotiales, Hypocreales and Fusarium sp., recorded mostly as endophytes.

Año de publicación:

2014

Keywords:

• HONGOS MICORRÍZICOS
• Biólogo – Disertaciones académicas.
• Orquídeas epífitas

Fuente:

rraae