Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast

Abstract:

The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial fructosyltransferase (LsdA) produced in Pichia pastoris was studied. The glycosylated enzyme behaved similarly to non-glycosylated LsdA when substrate specificity, fructo-oligosaccharide (FOS) production, sucrose hydrolysis or levan formation reactions were carried out under different experimental conditions. The kinetic parameters for native or yeast-expressed LsdA determined at 60°C, condition for the highest hydrolytic activity, followed a conventional Michaelis-Menten kinetics. Synthase activity of this levansucrase increased in water-restricted environments by addition of salt or organic solvent to the reaction mixtures.

Año de publicación:

2004

Keywords:

• Levansucrase
• N-glycosylation
• Fructosyltransferase
• Fructo-oligosaccharides
• Catalytical properties
• PICHIA PASTORIS

Fuente:

scopus