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Characterization of IS 6110 insertions in the dnaA-dnaN intergenic region of Mycobacterium tuberculosis clinical isolates

Abstract:

Mycobacterium tuberculosis isolates with identical IS 6110 restriction fragment length polymorphism (RFLP) patterns are considered to be clonally related. The presence of IS 6110 in the dnaA-dnaN intergenic region, one preferential locus for the integration of IS 6110, was evaluated in 125 M. tuberculosis isolates. Five isolates had IS 6110 inserted in this region, and two consisted of a mix of isogenic strains that putatively have evolved during a single infection. Strains from the same isolate had identical spoligo and mycobacterial interspersed repetitive unit-variable-number tandem repeat profiles, but had slight variations in IS 6110 RFLP patterns, due to the presence of IS 6110 in the dnaA-dnaN intergenic region. Duplication of the dnaA-dnaN intergenic region was found in one isogenic strain. © Journal compilation © 2009 European Society of Clinical Microbiology and Infectious Diseases.