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Characterization of Two Outbreaks of Vancomycin-Resistant Enterococcus Faecium in a Pediatric Care Center in Mexico City

Abstract:

Methods Our hospital is a Tertiary Care center, 244 beds facility. Two VRE outbreaks were reported from August 2009 to October 2009. The first outbreak occurred in the intensive care unit and the second in the oncology ward. We performed a retrospective study to describe the isolates. The identification of the species was based on conventional biochemical tests. Antimicrobial drug susceptibility pattern was obtained by Kirby-Bauer disk diffusion method and confirmed by minimum inhibitory concentration (MIC). A multiplex polymerase chain reaction (PCR) for detection of the genotype was used. The molecular characterization of VRE was performed by analysis of isolated DNA by pulse field gel electrophoresis (PFGE). The aim of this study was to determinate the strains relatedness and reinforce the application of preventive measures by health personnel. Results A total number of 14 strains obtained from five …

Año de publicación:

2012

Keywords:

    Fuente:

    googlegoogle

    Tipo de documento:

    Other

    Estado:

    Acceso abierto

    Áreas de conocimiento:

    • Infección
    • Microbiología
    • Microbiología

    Áreas temáticas:

    • Medicina forense; incidencia de enfermedades
    • Problemas sociales y servicios a grupos

    Contribuidores:

    Joselyn ZulaJoselyn Zula