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Chromosomal abnormalities are related to location and grade of osteoarthritis

Abstract:

Objective: To investigate the frequency of numerical aberrations of chromosomes 7, X and Y in patients with osteoarthritis (OA) by performing fluorescent in situ hybridization (FISH) studies on articular cartilage, and to correlate the chromosomal changes with the degree and location of articular involvement. Patients: Thirty-four women and 10 m en with OA were included in the study. As a control group, 6 women and 5 men operated for orthopedic disorders other than OA were analyzed. Methods: FISH studies were perfo rmed on hip or knee cartilage, using two-color centromere-specific probes for chromosomes 7 & X for women and 7 & Y for men. Results: FISH analysis revealed that 46% of OA p atients had numerical abnormalities of chromosomes 7, X or Y. An extra chromosome 7 (trisomy 7) was present in 35% of patients with chromosomal aberrations. All males with OA lost the Y chromosome while 15% of the women had loss of one chromosome X (monosomy X). Trisomy 7 was associated with hip OA (p = 0.019) and advanced OA according to the Kellgren and Lawrence classification (p = 0.05). None of the 11 controls showed abnormalities in the chromosomes analyzed. Conclusions: FISH analysis showed the pres ence of numerical chromosomal abnormalities in the articular cartilage of patients with OA. © 2004 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.