Comparative study of elongated and globose Woronin bodies using electron energy loss spectroscopy (EELS) and imaging (ESI)

Abstract:

Ultrastructure accompanied by electron energy loss spectroscopic data of septal structures in two species of Ascomycetes, Sarcosphaera crassa and Disciotis venosa, is described. The septal apparatus of both species appeared to be typical of members of the Pezizales. Spherical, slightly quadrangular or hexagonal Woronin bodies of S. crassa were characterized by the presence of increased amounts of nitrogen, sulphur and phosphorus compared with young cell walls and septal plug material. Similar amounts of calcium were observed in all structures, although not much nitrogen was detected in the septal plug. The rectangular, elongated, Woronin bodies present in mycelium isolated from fruit bodies, as well as in hyphae forming sporocarps of D. venosa, did not contain as much phosphorus and sulphur as the spherical bodies and septal plug material. The elongated Woronin bodies were also characterized by a higher level of calcium. The distribution of nitrogen in Woronin bodies and other elements in septal pore material were similar to those observed in S. crassa. The use of Swift's reaction revealed the proteinaceous nature of the rectangular bodies. © 1993, British Mycological Society. All rights reserved.

Año de publicación:

1993

Keywords:

Fuente:

scopus