Regresar

Cosmix-Plexing: A novel way to generate and use combinatorial phage-display libraries

Abstract:

This work describes the Cosmix-Plexing® procedure. It provides a novel way to use and generate combinatorial libraries of peptides displayed on the surface of filamentous phages. Using the Cosmix-Plexing approach, the left and right sections of the variable domain can be induced by the use of type II restriction enzymes. Thus, a population with "optimized" sequences for a larger number of amino acid residues can be generated. It can also simplify the production of extension libraries by the introduction of specific cassettes into existing libraries. This technology enabled us to efficiently isolate ligands having a great affinity for several target molecules of the EVH1 of Vesl domain from an initial library that was rich in proline.