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Covalent immobilization of lysozyme in silicone rubber modified by easy chemical grafting

Abstract:

Functionalization of silicone rubber films with lysozyme was achieved by grafting copolymerization and its chemical activation allowing the covalent immobilization of the enzyme. The new materials were characterized by means of Fourier-Transform infrared spectroscopy, differential scanning calorimetry, thermogravimetric analysis, contact angle, atomic force microscopy, and mechanical properties of films. The enzymatic activity of films was studied by a suspension of lyophilized Micrococcus lysodeikticus. The activity test was inquired at different pH and temperatures, exhibiting enzymatic activity 20 °C above the free lysozyme, and at pH = 5 where the free lysozyme did not show activity.

Año de publicación:

2017

Keywords:

    Fuente:

    scopusscopus

    Tipo de documento:

    Article

    Estado:

    Acceso restringido

    Áreas de conocimiento:

    • Biotecnología
    • Biomateriales
    • Bioquímica

    Áreas temáticas:

    • Tecnología de otros productos orgánicos

    Contribuidores:

    López‐saucedo F.López‐saucedo F.
    Isoshima T.Isoshima T.
    Emilio BucioEmilio Bucio
    Flores‐rojas G.G.Flores‐rojas G.G.