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Complexity and solutions to the isolation problem of Gram negative lipopolysaccharides' bacteria molecular species
ReviewAbstract: Gram negative bacteria frequently synthesize a great number of lipopolysaccharides (LPS) molecular sPalabras claves:chromatography, Electrophoresis, Elution, Isolation, lipopolysaccharides, Mass spectrometry, Reverse stainingAutores:Eugenio Hardy, Pupo E.Fuentes:scopus"Reverse-staining" of biomolecules in electrophoresis gels: Analytical and micropreparative applications
ReviewAbstract: Negative or reverse staining using imidazole and zinc salts for protein detection in electrophoresisPalabras claves:Gel electrophoresis, lipopolysaccharides, Negative staining of gels, Nucleic acids, proteins, Proteomic purification, Proteomic sample preparation, Reverse staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusDetection of PEGylated proteins in polyacrylamide gels by reverse staining with zinc and imidazole salts
ArticleAbstract: The reverse staining, with imidazole-SDS-zinc, of PEG-linked proteins separated by SDS-PAGE was studPalabras claves:Gel electrophoresis, PEGylated interferon, Protein staining, Reverse staining, Zinc stainingAutores:Báez R., Eugenio Hardy, Ramon J., Ruiz A., Saez V., Tejeda Y.Fuentes:scopusDetection of biomolecules in electrophoresis gels with salts of imidazole and zinc II: A decade of research
ReviewAbstract: The proven ability of gel electrophoresis to simultaneously resolve, in a single experiment, many coPalabras claves:Gel electrophoresis detection, Imidazole zinc staining, lipopolysaccharides, Nucleic acids, proteins, Reverse staining, ReviewAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusMatrix-assisted laser desorption/ionization-time of flight-mass spectrometry of lipopolysaccharide species separated by slab-polyacrylamide gel electrophoresis: High-resolution separation and molecular weight determination of lipooligo-saccharides from Vibro fischeri strain HMK
ArticleAbstract: We recently demonstrated that the combined use of lipopolysaccharide (LPS) reverse staining and highPalabras claves:Elution, Gel electrophoresis, LIPOPOLYSACCHARIDE, Mass spectrometry, Reverse stainingAutores:Apicella M.A., Eugenio Hardy, Gibson B.W., Phillips N.J., Pupo E.Fuentes:scopusSingle‐step electrotransfer of reverse‐stained proteins from sodium dodecyl sulfate‐polyacrylamide gel onto reversed‐phase minicartridge and subsequent desalting and elution with a conventional high‐performance liquid chromatography gradient system for analysis
ArticleAbstract: Isolation of proteins from polyacrylamide electrophoresis gels by a novel combination of techniquesPalabras claves:High‐performance liquid chromatography, Protein microanalysis, Reverse staining, Sodium dodecyl sulfate‐polyacrylamide gel electrophoresisAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Frank R., Madrazo J., Mendez E.Fuentes:scopus