Mostrando 10 resultados de: 67
Publisher
Biotecnologia Aplicada(12)
Electrophoresis(10)
Analytical Biochemistry(9)
Biomolecules(2)
Frontiers in Physiology(2)
Área temáticas
Bioquímica(25)
Farmacología y terapéutica(24)
Microorganismos, hongos y algas(24)
Enfermedades(14)
Fisiología humana(12)
High yield elution of proteins from sodium dodecyl sulfate- polyacrylamide gels at the low-picomole level. Application to N-terminal sequencing of a scarce protein and to in-solution biological activity analysis of on-gel renatured proteins
Conference ObjectAbstract: A simple, reliable procedure for practically quantitative (90-98%) and fast (<30 min) elution of proPalabras claves:Passive elution, Protein detection, Protein micropurification, SDS PAGE, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V., Santana-Milián H.Fuentes:scopusHigh-efficiency passive elution of bacterial lipopolysaccharides from polyacrylamide gels
ArticleAbstract: We recently described a method for recovering polyacrylamide-gel- separated bacterial lipopolysacchaPalabras claves:Gel electrophoresis, lipopolysaccharides, purification, Silver staining, Zinc stainingAutores:Alonso M., Eugenio Hardy, López C.M., Pupo E.Fuentes:scopusElution of lipopolysaccharides from polyacrylamide gels
ArticleAbstract: A simple procedure for elution in water of bacterial lipopolysaccharides (LPS) from sodium dodecyl sPalabras claves:Elution, Gel electrophoresis, LAL, Silver staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio Hardy, Guerra M., Pupo E., Santana-Milián H.Fuentes:scopusElution of unmodified oligodeoxynucleotides from zinc-Imidazole negatively stained polyacrylamide gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Coizeau E., Eugenio Hardy, Pupo E., Silva J.A., Silva R.Fuentes:scopusEmerging pathways of communication between the heart and non-cardiac organs
ArticleAbstract: The breakthrough discovery of cardiac natriuretic peptides provided the first direct demonstration oPalabras claves:heart, inflammation, Liver, Matrix metalloproteinase, metabolism, microRNA, Natriuretic peptidesAutores:Eugenio Hardy, Fernandez‐Patron C.Fuentes:scopusEnhanced ELISA sensitivity using TCA for efficient coating of biologically active lipopolysaccharides or lipid A to the solid phase
ArticleAbstract: A new simple, reproducible and sensitive ELISA that uses trichloroacetic acid (TCA) for the coatingPalabras claves:Coating efficiency, Elisa, Lipid A, LIPOPOLYSACCHARIDE, Trichloroacetic acid ([76-03-9])Autores:Eugenio Hardy, Llano M., Ohlin M.Fuentes:scopusExpression and folding of an interleukin-2-proinsulin fusion protein and its conversion into insulin by a single step enzymatic removal of the C-peptide and the N-terminal fused sequence
ArticleAbstract: We report the expression in E. coli of a proinsulin fusion protein carrying a modified interleukin-2Palabras claves:Enzymatic processing, Proinsulin folding, Recombinant insulinAutores:Besada-Pérez V., Castellanos‐Serra L., Eugenio Hardy, Falcón-Cama V., Fernandez C., Gonzalez M., Herrera L., Perez G., Santiago Nelson Vispo, Santos-Savio A., Silva A., Ubieta R.Fuentes:scopusExpression of il-2/proinsulin fusion protein: Folding of the fusion protein and conversion into insulin by a one step removal of c-peptide and leader sequence
ArticleAbstract:Palabras claves:Autores:Besada-Pérez V., Castellanos L.R., Estrada R., Eugenio Hardy, Fernandez C., Gonzalez M., Herrera L., Jimenez V., Perez G., Santiago Nelson Vispo, Silva A., Ubieta R.Fuentes:scopusFast passive elution of DNA from zinc-imidazole negatively stained polyacrylamide gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Sánchez J.C.Fuentes:scopusFeasibility, via a new interface, of mass spectrometry of lipopolysaccharides separated in polyacrylamide gels
ArticleAbstract: For the first time, it was demonstrated the feasibility of analyzing by mass spectrometry (MS) Gram-Palabras claves:Autores:Eugenio Hardy, Pupo E.Fuentes:scopus