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Complexity and solutions to the isolation problem of Gram negative lipopolysaccharides' bacteria molecular species
ReviewAbstract: Gram negative bacteria frequently synthesize a great number of lipopolysaccharides (LPS) molecular sPalabras claves:chromatography, Electrophoresis, Elution, Isolation, lipopolysaccharides, Mass spectrometry, Reverse stainingAutores:Eugenio Hardy, Pupo E.Fuentes:scopus"Reverse-staining" of biomolecules in electrophoresis gels: Analytical and micropreparative applications
ReviewAbstract: Negative or reverse staining using imidazole and zinc salts for protein detection in electrophoresisPalabras claves:Gel electrophoresis, lipopolysaccharides, Negative staining of gels, Nucleic acids, proteins, Proteomic purification, Proteomic sample preparation, Reverse staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusDetection of biomolecules in electrophoresis gels with salts of imidazole and zinc II: A decade of research
ReviewAbstract: The proven ability of gel electrophoresis to simultaneously resolve, in a single experiment, many coPalabras claves:Gel electrophoresis detection, Imidazole zinc staining, lipopolysaccharides, Nucleic acids, proteins, Reverse staining, ReviewAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusNanoaggregates of micropurified lipopolysaccharide identified using dynamic light scattering, zeta potential measurement, and TLR4 signaling activity
ArticleAbstract: Nanoaggregates composed of selected glycoforms from Escherichia coli 055:B5 lipopolysaccharide (LPS)Palabras claves:Aggregates, dynamic light scattering, Gel electrophoresis, lipopolysaccharides, TLR4, Zeta potentialAutores:Eugenio Hardy, Japaridze A., Kamphuis T., Wilschut J.C., Winterhalter M.Fuentes:scopusHigh-efficiency passive elution of bacterial lipopolysaccharides from polyacrylamide gels
ArticleAbstract: We recently described a method for recovering polyacrylamide-gel- separated bacterial lipopolysacchaPalabras claves:Gel electrophoresis, lipopolysaccharides, purification, Silver staining, Zinc stainingAutores:Alonso M., Eugenio Hardy, López C.M., Pupo E.Fuentes:scopusMice immunization with gel electrophoresis-micropurified bacterial lipopolysaccharides
ArticleAbstract: Some evidence on the possible use of sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDSPalabras claves:Elution, Gel electrophoresis, Immunization, lipopolysaccharides, Zinc stainingAutores:Aguila A., Castellanos‐Serra L., Eugenio Hardy, Núñez J.F., Pupo E., Santana-Milián H.Fuentes:scopusLipopolysaccharide (LPS) and protein-LPS complexes: Detection and characterization by gel electrophoresis, mass spectrometry and bioassays
ArticleAbstract: A prerequisite to the discovery and characterization of lipopolysaccharide (LPS) interaction with spPalabras claves:Agarose, Aggregates, Electrophoresis, ESI-MS, lipopolysaccharides, MALDITOF-MS, Mass spectrometry, TLR4, Zinc stainAutores:Eugenio Hardy, Luis E. Trujillo, Rodriguez C.Fuentes:scopusLipopolysaccharide aggregates in native agarose gels detected by reversible negative staining with imidazole and zinc salts
ArticleAbstract: We investigated the use of imidazole and zinc salts for the detection of lipopolysaccharide (LPS) agPalabras claves:Agarose, Aggregates, Cationic proteins, Electrophoresis, lipopolysaccharides, Zinc stainAutores:Eugenio Hardy, Rodriguez C.Fuentes:scopusSelective precipitation of zinc imidazolate: Application to the detection of biomolecules (proteins, nucleic acids, and bacterial glycolipids) separated in polyacrylamide gels
ArticleAbstract: At the start of the past decade a group of scientists from the Center for Genetic Engineering and BiPalabras claves:Electrophoresis, lipopolysaccharides, Negative staining, Nucleic acids, Polyacrylamide, proteinsAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopus