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"Reverse-staining" of biomolecules in electrophoresis gels: Analytical and micropreparative applications
ReviewAbstract: Negative or reverse staining using imidazole and zinc salts for protein detection in electrophoresisPalabras claves:Gel electrophoresis, lipopolysaccharides, Negative staining of gels, Nucleic acids, proteins, Proteomic purification, Proteomic sample preparation, Reverse staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusFast passive elution of DNA from zinc-imidazole negatively stained polyacrylamide gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Sánchez J.C.Fuentes:scopusElution of lipopolysaccharides from polyacrylamide gels
ArticleAbstract: A simple procedure for elution in water of bacterial lipopolysaccharides (LPS) from sodium dodecyl sPalabras claves:Elution, Gel electrophoresis, LAL, Silver staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio Hardy, Guerra M., Pupo E., Santana-Milián H.Fuentes:scopusElution of unmodified oligodeoxynucleotides from zinc-Imidazole negatively stained polyacrylamide gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Coizeau E., Eugenio Hardy, Pupo E., Silva J.A., Silva R.Fuentes:scopusDouble Staining of Coomassie Blue-Stained Polyacrylamide Gels by Imidazole-Sodium Dodecyl Sulfate-Zinc Reverse Staining: Sensitive Detection of Coomassie Blue-Undetected Proteins
ArticleAbstract: The sensitivity, simplicity, and relative rapidity of Coomassie blue staining have made this techniqPalabras claves:Autores:Castellanos L.R., Eugenio Hardy, Fernandez‐Patron C., Seoane J., Sosa A.E.Fuentes:scopusNanoaggregates of micropurified lipopolysaccharide identified using dynamic light scattering, zeta potential measurement, and TLR4 signaling activity
ArticleAbstract: Nanoaggregates composed of selected glycoforms from Escherichia coli 055:B5 lipopolysaccharide (LPS)Palabras claves:Aggregates, dynamic light scattering, Gel electrophoresis, lipopolysaccharides, TLR4, Zeta potentialAutores:Eugenio Hardy, Japaridze A., Kamphuis T., Wilschut J.C., Winterhalter M.Fuentes:scopusLipopolysaccharide aggregates in native agarose gels detected by reversible negative staining with imidazole and zinc salts
ArticleAbstract: We investigated the use of imidazole and zinc salts for the detection of lipopolysaccharide (LPS) agPalabras claves:Agarose, Aggregates, Cationic proteins, Electrophoresis, lipopolysaccharides, Zinc stainAutores:Eugenio Hardy, Rodriguez C.Fuentes:scopusSensitive reverse staining of bacterial lipopolysaccharides on polyacrylamide gels by using zinc and imidazole salts
ArticleAbstract: We present a new method for visualizing bacterial lipopolysaccharides (LPS)/lipooligosaccharides (LOPalabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Pupo E., Reyes J.Fuentes:scopusRecovery of biologically active proteins detected with imidazole-sodium dodecyl sulfate-zinc (reverse stain) on sodium dodecyl sulfate gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Hernandez L., Santana-Milián H., Sosa A.E.Fuentes:scopus