Mostrando 10 resultados de: 10
Filtros aplicados
Área temáticas
Bioquímica(7)
Microorganismos, hongos y algas(4)
Química analítica(2)
Biología(1)
Farmacología y terapéutica(1)
Área de conocimiento
Bioquímica(7)
Microbiología(3)
Biología molecular(2)
Biomedicina(2)
Espectrometría de masas(1)
Origen
scopus(10)
A procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
ArticleAbstract: We developed a technique that allows rapid protein elution from polyacrylamide gel bands at room temPalabras claves:Passive elution, Protein detection, Protein micropurification, Sodium dodecyl, Sulfate-polyacrylamide gel electrophoresis, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V.Fuentes:scopusDetection of PEGylated proteins in polyacrylamide gels by reverse staining with zinc and imidazole salts
ArticleAbstract: The reverse staining, with imidazole-SDS-zinc, of PEG-linked proteins separated by SDS-PAGE was studPalabras claves:Gel electrophoresis, PEGylated interferon, Protein staining, Reverse staining, Zinc stainingAutores:Báez R., Eugenio Hardy, Ramon J., Ruiz A., Saez V., Tejeda Y.Fuentes:scopusDetection of biomolecules in electrophoresis gels with salts of imidazole and zinc II: A decade of research
ReviewAbstract: The proven ability of gel electrophoresis to simultaneously resolve, in a single experiment, many coPalabras claves:Gel electrophoresis detection, Imidazole zinc staining, lipopolysaccharides, Nucleic acids, proteins, Reverse staining, ReviewAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusNegative staining with zinc-imidazole of gel electrophoresis-separated nucleic acids
ArticleAbstract: Zinc and imidazole salts were applied for the detection of nucleic acids on either polyacrylamide orPalabras claves:Agarose gel electrophoresis, Detection of nucleic acids, Negative staining, Zinc staining, Zinc-imidazole detectionAutores:Casalvilla R., Castellanos‐Serra L., Eugenio Hardy, López E., Luis E. Trujillo, Pupo E., Sosa A.E.Fuentes:scopusHigh-efficiency passive elution of bacterial lipopolysaccharides from polyacrylamide gels
ArticleAbstract: We recently described a method for recovering polyacrylamide-gel- separated bacterial lipopolysacchaPalabras claves:Gel electrophoresis, lipopolysaccharides, purification, Silver staining, Zinc stainingAutores:Alonso M., Eugenio Hardy, López C.M., Pupo E.Fuentes:scopusMatrix-assisted laser desorption/ionization-time of flight-mass spectrometry of lipopolysaccharide species separated by slab-polyacrylamide gel electrophoresis: High-resolution separation and molecular weight determination of lipooligo-saccharides from Vibro fischeri strain HMK
ArticleAbstract: We recently demonstrated that the combined use of lipopolysaccharide (LPS) reverse staining and highPalabras claves:Elution, Gel electrophoresis, LIPOPOLYSACCHARIDE, Mass spectrometry, Reverse stainingAutores:Apicella M.A., Eugenio Hardy, Gibson B.W., Phillips N.J., Pupo E.Fuentes:scopusMice immunization with gel electrophoresis-micropurified bacterial lipopolysaccharides
ArticleAbstract: Some evidence on the possible use of sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDSPalabras claves:Elution, Gel electrophoresis, Immunization, lipopolysaccharides, Zinc stainingAutores:Aguila A., Castellanos‐Serra L., Eugenio Hardy, Núñez J.F., Pupo E., Santana-Milián H.Fuentes:scopusIsolation of smooth-type lipopolysaccharides to electrophoretic homogeneity
ArticleAbstract: The high structural heterogeneity of smooth-type lipopolysaccharides (LPS) enormously complicates thPalabras claves:biological activity, Limulus amebocyte lysate, LIPOPOLYSACCHARIDEAutores:Eugenio Hardy, Pupo E.Fuentes:scopusUnderstanding the mechanism of the zinc-ion stains of biomacromolecules in electrophoresis gels: Generalization of the reverse-staining technique
ArticleAbstract: We have recently shown that a few nanograms of protein separated by electrophoresis in sodium dodecyPalabras claves:detection, Imidazole, inflammation, Lysozyme, Negative stainingAutores:Castellanos‐Serra L., Estevez E., Eugenio Hardy, Fernandez‐Patron C., Frank R., Guerra M., Mehl E.Fuentes:scopusZinc-imidazole positive: A new method for DNA detection after electrophoresis on agarose gels not interfering with DNA biological integrity
ArticleAbstract: Nucleic acids separated by gel electrophoresis are commonly detected within the gel matrix with ethiPalabras claves:Detection of nucleic acids, DNA staining, Gel electrophoresis, UV damage, Zinc stainingAutores:Casalvilla R., Eugenio Hardy, Fernandez‐Patron C., Pupo E., Sosa A.E.Fuentes:scopus