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Methods in Molecular Biology(3)
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Microorganismos, hongos y algas(6)
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scopus(15)
Changes of defense proteins in the extracellular proteome of grapevine (Vitis vinifera cv. Gamay) cell cultures in response to elicitors
ArticleAbstract: In plant cells, elicitors induce defense responses that resemble those triggered by pathogen attack,Palabras claves:2-DE, cell culture, Chitinase-III, Class III secretory basic peroxidase, Extracellular proteome, GrapevineAutores:Martínez R., Martínez-Esteso M.J., Pedreño M.A., Susana Sellés-Marchart, Vera-Urbina J.C.Fuentes:scopusA focused multiple reaction monitoring (MRM) quantitative method for bioactive grapevine stilbenes by ultra-high-performance liquid chromatography coupled to triple-quadrupole mass spectrometry (UHPLC-QqQ)
ArticleAbstract: Grapevine stilbenes are a family of polyphenols which derive from trans-resveratrol having antifungaPalabras claves:BIOCONVERSIÓN, cell culture, Epsilon-viniferin, MRM, Piceatannol, Piceid, Pterostilbene, Resveratrol, UHPLC, wineAutores:Hurtado-Gaitán E., Martínez R., Martínez-Márquez A., Samper-Herrero A., Susana Sellés-MarchartFuentes:scopusA tau class glutathione-S-transferase is involved in trans-resveratrol transport out of grapevine cells
ArticleAbstract: Vitis vinifera cell cultures respond to pathogens and elicitors by synthesizing and extracellularlyPalabras claves:cell culture, DIGE proteomics, Glutathione-S-transferase, qRT-PCR, Resveratrol, Transformation, transport, Vitis viníferaAutores:Hurtado E., Jaime Morante Carriel, Martínez R., Martínez-Esteso M.J., Martínez-Márquez A., Palazon J., Susana Sellés-Marchart, Vilella-Antón M.T.Fuentes:scopusDevelopment and validation of MRM methods to quantify protein isoforms of polyphenol oxidase in loquat fruits
ArticleAbstract: Multiple reaction monitoring (MRM) is emerging as a promising technique for the detection and quantiPalabras claves:Isoform, loquat fruits, Multiple reaction monitoring, polyphenol oxidase, transitionsAutores:Jaime Morante Carriel, Luque I., Martínez R., Martínez-Esteso M.J., Martínez-Márquez A., Pineda-Lucas J.L., Susana Sellés-MarchartFuentes:scopusDIGE analysis of proteome changes accompanying large resveratrol production by grapevine (Vitis vinifera cv. Gamay) cell cultures in response to methyl-β-cyclodextrin and methyl jasmonate elicitors
ArticleAbstract: We had previously shown that Vitis vinifera cv. Gamay cell suspension accumulates extracellularly laPalabras claves:cell culture, Cyclodextrin, DIGE, Glutathione-S transferase class tau, Grapevine, Methyl jasmonate, Proteome, Resveratrol, Stilbene synthaseAutores:Martínez R., Martínez-Esteso M.J., Pedreño M.A., Susana Sellés-Marchart, Vera-Urbina J.C.Fuentes:scopusEffect of detergents, trypsin and unsaturated fatty acids on latent loquat fruit polyphenol oxidase: Basis for the enzyme's activity regulation
ArticleAbstract: The effects of detergents, trypsin and fatty acids on structural and functional properties of a purePalabras claves:Alkyl sulfates, Alkyl sulfonates, Chlorogenic acid, Fluorescence, latency, Linolenic acid, Loquat fruit, polyphenol oxidase, regulation, TrypsinAutores:Casado-Vela J., Martínez R., Susana Sellés-MarchartFuentes:scopusDifferential plant proteome analysis by isobaric tags for relative and absolute quantitation (iTRAQ)
ArticleAbstract: Protein relative quantitation is one of the main targets in many proteomic experiments. Among the raPalabras claves:Isobaric tag, iTRAQ, Liquid chromatography, Mass spectrometry, Plant proteomics, Quantitation, Strong cation exchangeAutores:Casado-Vela J., Martínez R., Martínez-Esteso M.J., Pedreño M.A., Susana Sellés-MarchartFuentes:scopusITRAQ-based profiling of grape berry exocarp proteins during ripening using a parallel mass spectrometric method
ArticleAbstract: The 4-plex iTRAQ platform was utilized to analyze the protein profiles in four stages of grapevine bPalabras claves:Autores:Casado-Vela J., Elortza F., Martínez R., Martínez-Esteso M.J., Pedreño M.A., Susana Sellés-MarchartFuentes:scopusIsolation of a latent polyphenol oxidase from loquat fruit (Eriobotrya japonica Lindl.): Kinetic characterization and comparison with the active form
ArticleAbstract: Polyphenol oxidase (PPO) has been extracted from both soluble and particulate fractions of loquat frPalabras claves:Chlorogenic acid, Kinetic characterization, latency, Loquat fruit, Membrane proteins, polyphenol oxidase, purification, SDS, Triton X-114Autores:Casado-Vela J., Martínez R., Susana Sellés-MarchartFuentes:scopusRNA isolation from loquat and other recalcitrant woody plants with high quality and yield
ArticleAbstract: RNA isolation is difficult in plants that contain large amounts of polysaccharides and polyphenol coPalabras claves:fruits, Polyphenols, polysaccharides, RNA isolation, Woody plantsAutores:Jaime Morante Carriel, Luque I., Martínez R., Martínez-Esteso M.J., Martínez-Márquez A., Susana Sellés-MarchartFuentes:scopus