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Constitutive expression of enzymatically active Gluconacetobacter diazotrophicus levansucrase in the methylothrophic yeast Pichia pastoris
ArticleAbstract: Gluconacetobacter diazotrophicus levansucrase (LsdA) was constitutively expressed in P. pastoris undPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Glyceraldehyde-3-phosphate dehydrogenase promoter, Levansucrase, PICHIA PASTORISAutores:Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Sotolongo M., Támbara Y.Fuentes:googlescopusFunctional production and secretion of the Gluconacetobacter diazotrophicus fructose-releasing exo-levanase (LsdB) in Pichia pastoris
ArticleAbstract: The gene encoding the fructose-releasing exo-levanase (LsdB; EC 3.2.1.65) from Gluconacetobacter diaPalabras claves:Alcohol oxidase, AOX, AOX1 promoter, Gap, GAP promoter, Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus levanase, Gluconacetobacter diazotrophicus levansucrase, Glyceraldehyde 3-phosphate dehydrogenase, Levanase, LsdA, LsdB, PICHIA PASTORISAutores:Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Menéndez C.Fuentes:googlescopusImproving the expression of mini-proinsulin in Pichia pastoris
ArticleAbstract: Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achiPalabras claves:AOX promoter, fed-batch culture, PICHIA PASTORIS, Recombinant human insulinAutores:Besada-Pérez V., Cabello C., Castellanos‐Serra L., Del C. Abrahantes-Pérez M., Del Toro Y., García Y., Hernández L., Hidalgo A., José-Manuel Pais-Chanfrau, Licor L., Mansur M., Martinez E., Plana L., Támbara Y., Valdés J.Fuentes:googlescopusMultiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris
ArticleAbstract: We have found a direct relationship between protein production in Pichia pastoris and the number ofPalabras claves:Insulin, Multiple gene copies, PICHIA PASTORIS, Protein expressionAutores:Besada-Pérez V., Cabello C., Castellanos L.R., García L., Hernández L., Hidalgo A., José-Manuel Pais-Chanfrau, Lamazares E., Mansur M., Martinez E., Plana L., Terrero Y., Valdés J., Varas L.Fuentes:googlescopusOptimization of culture medium for large-scale production of heterologous proteins in Pichia pastoris to be used in nanoscience and other biotechnological fields
ArticleAbstract: The methylotrophic yeast Pichia pastoris became an excellent and successfully expression system forPalabras claves:Chemical defined medium, Heterologous protein expression system, PICHIA PASTORISAutores:José-Manuel Pais-Chanfrau, Luis E. TrujilloFuentes:googlescopusModeling of mini-proinsulin production in Pichia pastoris using the AOX promoter
ArticleAbstract: An unstructured model based on mass balance equations for a recombinant methylotrophic yeast PichiaPalabras claves:fed-batch culture, Fermentation, Modeling, PICHIA PASTORIS, Recombinant human insulinAutores:Cabello C., José-Manuel Pais-Chanfrau, Mansur M., Rodríguez L., Valdés J., Varas L.Fuentes:googlescopus