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A new area of application and research in bio-processes: Biotechnologies in civil construction
ReviewAbstract: Construction Biotechnology is a new scientific and engineering discipline that has been developing ePalabras claves:BIOGENIC, Biomaterials, Biotechnological, cement, concrete, MicroorganismsAutores:A. Barberán, A. Cajas, D. Chávez, José-Manuel Pais-Chanfrau, Luis E. Trujillo, M. C. Egas, M. Criollo, Pineda J.Fuentes:scopusFunctional production and secretion of the Gluconacetobacter diazotrophicus fructose-releasing exo-levanase (LsdB) in Pichia pastoris
ArticleAbstract: The gene encoding the fructose-releasing exo-levanase (LsdB; EC 3.2.1.65) from Gluconacetobacter diaPalabras claves:Alcohol oxidase, AOX, AOX1 promoter, Gap, GAP promoter, Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus levanase, Gluconacetobacter diazotrophicus levansucrase, Glyceraldehyde 3-phosphate dehydrogenase, Levanase, LsdA, LsdB, PICHIA PASTORISAutores:Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Menéndez C.Fuentes:googlescopusImproving the expression of mini-proinsulin in Pichia pastoris
ArticleAbstract: Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achiPalabras claves:AOX promoter, fed-batch culture, PICHIA PASTORIS, Recombinant human insulinAutores:Besada-Pérez V., Cabello C., Castellanos‐Serra L., Del C. Abrahantes-Pérez M., Del Toro Y., García Y., Hernández L., Hidalgo A., José-Manuel Pais-Chanfrau, Licor L., Mansur M., Martinez E., Plana L., Támbara Y., Valdés J.Fuentes:googlescopusIdentification and recombinant expression of a bacterial exolevanase useful for the production of high fructose syrups
ArticleAbstract: The sugarcane endophyte Gluconacetobacter diazotrophicus produces levan from sucrose by a secreted lPalabras claves:Autores:Alfonso D., Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Menéndez C., Ramírez R.Fuentes:googlescopusThe impact of disposables on project economics in a new antibody plant: A case study
ReviewAbstract: This paper compares two project alternatives for the construction of a new 100 kg antibody plant, baPalabras claves:Autores:Chico E., José-Manuel Pais-Chanfrau, Zorrilla K.Fuentes:googlescopusMulti-Objective Optimization of Beverage Based on Lactic Fermentation of Goat’s Milk Whey and Fruit Juice Mixes by Kefir Granules
ArticleAbstract: Numerous fruits are produced in Ecuador, of which about 40% are never eaten. In addition, fresh goatPalabras claves:common strawberry (Fragaria × ananassa), functional beverage, goat milk whey valorisation, multi-objective optimization, Response Surface Methodology, tree-tomato (Solanum betaceum)Autores:Diana Alexandra Nastar Marcillo, Jimmy Nuñez, Jimmy Núñez-Pérez, José-Manuel Pais-Chanfrau, Nicolás Sebastián Pinto Mosquera, Rosario C. Espín-Valladares, Valeria Olmedo-GalarzaFuentes:googlescopusMulti-Objective Statistical Optimization of Pectinolytic Enzymes Production by an Aspergillus sp. on Dehydrated Coffee Residues in Solid-State Fermentation
ArticleAbstract: Pectinolytic enzymes are a group of enzymes widely used in the food industry. They can be obtained tPalabras claves:Aspergillus sp, coffee residues, pectinolytic enzymes, Response Surface Methodology, solid-state fermentationAutores:Brayan Santiago Chávez Arias, Jimmy Nuñez, Jimmy Núñez-Pérez, José-Manuel Pais-Chanfrau, Juan Carlos De la Vega Quintero, Santiago Zárate BacaFuentes:googlescopusMultiple-objective optimization of lactic-fermentation parameters to obtain a functional-beverage candidate
ArticleAbstract: Background: Whey is the most abundant by-product of the cheese industry. It is estimated that it conPalabras claves:Central-composite design, Cheese industry, Fermented milk drink, Functional-beverage, Kefir grains, lactic acid bacteria, Lactic fermentation, Response Surface Methodology, Whey powder, YeastAutores:Jimmy Nuñez, Jimmy Núñez-Pérez, José-Manuel Pais-Chanfrau, Paola M. Alvarado-Cóndor, Rosario C. Espín-ValladaresFuentes:googlescopusOptimization of culture medium for large-scale production of heterologous proteins in Pichia pastoris to be used in nanoscience and other biotechnological fields
ArticleAbstract: The methylotrophic yeast Pichia pastoris became an excellent and successfully expression system forPalabras claves:Chemical defined medium, Heterologous protein expression system, PICHIA PASTORISAutores:José-Manuel Pais-Chanfrau, Luis E. TrujilloFuentes:googlescopusResponse surface methodology to optimize a bioprocess for kefiran production
ArticleAbstract: Kefiran is an edible biopolymer formed by a galactose and glucose chain in nearly equal proportions.Palabras claves:central composite design, Kefir granules, Kefiran, Whey powderAutores:Jimmy Cuaran-Guerrero, Jimmy Nuñez, Jimmy Núñez Pérez, José-Manuel Pais-Chanfrau, Leonor Margarita Rivera Intriago, Luis E. Trujillo, Milton Jimmy Cuaran Guerrero, Paola Margarita Alvarado CóndorFuentes:googlescopus