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scopus(17)
Constitutive expression of enzymatically active Gluconacetobacter diazotrophicus levansucrase in the methylothrophic yeast Pichia pastoris
ArticleAbstract: Gluconacetobacter diazotrophicus levansucrase (LsdA) was constitutively expressed in P. pastoris undPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Glyceraldehyde-3-phosphate dehydrogenase promoter, Levansucrase, PICHIA PASTORISAutores:Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Sotolongo M., Támbara Y.Fuentes:googlescopusFunctional production and secretion of the Gluconacetobacter diazotrophicus fructose-releasing exo-levanase (LsdB) in Pichia pastoris
ArticleAbstract: The gene encoding the fructose-releasing exo-levanase (LsdB; EC 3.2.1.65) from Gluconacetobacter diaPalabras claves:Alcohol oxidase, AOX, AOX1 promoter, Gap, GAP promoter, Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus levanase, Gluconacetobacter diazotrophicus levansucrase, Glyceraldehyde 3-phosphate dehydrogenase, Levanase, LsdA, LsdB, PICHIA PASTORISAutores:Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Menéndez C.Fuentes:googlescopusImproving the expression of mini-proinsulin in Pichia pastoris
ArticleAbstract: Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achiPalabras claves:AOX promoter, fed-batch culture, PICHIA PASTORIS, Recombinant human insulinAutores:Besada-Pérez V., Cabello C., Castellanos‐Serra L., Del C. Abrahantes-Pérez M., Del Toro Y., García Y., Hernández L., Hidalgo A., José-Manuel Pais-Chanfrau, Licor L., Mansur M., Martinez E., Plana L., Támbara Y., Valdés J.Fuentes:googlescopusIdentification and recombinant expression of a bacterial exolevanase useful for the production of high fructose syrups
ArticleAbstract: The sugarcane endophyte Gluconacetobacter diazotrophicus produces levan from sucrose by a secreted lPalabras claves:Autores:Alfonso D., Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Menéndez C., Ramírez R.Fuentes:googlescopusStatistical optimization of immunoaffinity purification of hepatitis B surface antigen using response surface methodology
ArticleAbstract: Response surface methodology (RSM) was successfully applied to find an elution condition enhancing tPalabras claves:Affinity, chromatography, Hepatitis B surface antigen, monoclonal antibodies, Optimization, Response surfaces methodologyAutores:José-Manuel Pais-Chanfrau, Linares-Domínguez M., López-Larraburo I., Márquez-Perera G., Masso-Rodríguez Y., Pestana-Vila Y., Sánchez-Romeu J.Fuentes:googlescopusThe extended methylene blue reduction test and milk quality
ArticleAbstract: Introduction. This study aimed to evaluate the quality of milk produced by six cattlemen's associatiPalabras claves:dairy cattle, Dairy industry, Microorganisms, Raw milk, somatic cellsAutores:Edmundo Recalde-Posso, Holger Pineda-Flores, Holguer Pineda-Flores, Jimmy Cuaran-Guerrero, Jimmy Nuñez, Jimmy Núñez-Pérez, José-Manuel Pais-Chanfrau, Lucía Yépez-Vásquez, Luis E. Trujillo, Mayra Pérez-Lomas, Milton J. Cuaran-Guerrero, Rosario C. Espín-ValladaresFuentes:googlescopusThe impact of disposables on project economics in a new antibody plant: A case study
ReviewAbstract: This paper compares two project alternatives for the construction of a new 100 kg antibody plant, baPalabras claves:Autores:Chico E., José-Manuel Pais-Chanfrau, Zorrilla K.Fuentes:googlescopusMulti-Objective Optimization of Beverage Based on Lactic Fermentation of Goat’s Milk Whey and Fruit Juice Mixes by Kefir Granules
ArticleAbstract: Numerous fruits are produced in Ecuador, of which about 40% are never eaten. In addition, fresh goatPalabras claves:common strawberry (Fragaria × ananassa), functional beverage, goat milk whey valorisation, multi-objective optimization, Response Surface Methodology, tree-tomato (Solanum betaceum)Autores:Diana Alexandra Nastar Marcillo, Jimmy Nuñez, Jimmy Núñez-Pérez, José-Manuel Pais-Chanfrau, Nicolás Sebastián Pinto Mosquera, Rosario C. Espín-Valladares, Valeria Olmedo-GalarzaFuentes:googlescopusMulti-Objective Statistical Optimization of Pectinolytic Enzymes Production by an Aspergillus sp. on Dehydrated Coffee Residues in Solid-State Fermentation
ArticleAbstract: Pectinolytic enzymes are a group of enzymes widely used in the food industry. They can be obtained tPalabras claves:Aspergillus sp, coffee residues, pectinolytic enzymes, Response Surface Methodology, solid-state fermentationAutores:Brayan Santiago Chávez Arias, Jimmy Nuñez, Jimmy Núñez-Pérez, José-Manuel Pais-Chanfrau, Juan Carlos De la Vega Quintero, Santiago Zárate BacaFuentes:googlescopusMultiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris
ArticleAbstract: We have found a direct relationship between protein production in Pichia pastoris and the number ofPalabras claves:Insulin, Multiple gene copies, PICHIA PASTORIS, Protein expressionAutores:Besada-Pérez V., Cabello C., Castellanos L.R., García L., Hernández L., Hidalgo A., José-Manuel Pais-Chanfrau, Lamazares E., Mansur M., Martinez E., Plana L., Terrero Y., Valdés J., Varas L.Fuentes:googlescopus