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Constitutive expression of enzymatically active Gluconacetobacter diazotrophicus levansucrase in the methylothrophic yeast Pichia pastoris
ArticleAbstract: Gluconacetobacter diazotrophicus levansucrase (LsdA) was constitutively expressed in P. pastoris undPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Glyceraldehyde-3-phosphate dehydrogenase promoter, Levansucrase, PICHIA PASTORISAutores:Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Sotolongo M., Támbara Y.Fuentes:googlescopusFunctional production and secretion of the Gluconacetobacter diazotrophicus fructose-releasing exo-levanase (LsdB) in Pichia pastoris
ArticleAbstract: The gene encoding the fructose-releasing exo-levanase (LsdB; EC 3.2.1.65) from Gluconacetobacter diaPalabras claves:Alcohol oxidase, AOX, AOX1 promoter, Gap, GAP promoter, Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus levanase, Gluconacetobacter diazotrophicus levansucrase, Glyceraldehyde 3-phosphate dehydrogenase, Levanase, LsdA, LsdB, PICHIA PASTORISAutores:Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Menéndez C.Fuentes:googlescopusMulti-Objective Optimization of Beverage Based on Lactic Fermentation of Goat’s Milk Whey and Fruit Juice Mixes by Kefir Granules
ArticleAbstract: Numerous fruits are produced in Ecuador, of which about 40% are never eaten. In addition, fresh goatPalabras claves:common strawberry (Fragaria × ananassa), functional beverage, goat milk whey valorisation, multi-objective optimization, Response Surface Methodology, tree-tomato (Solanum betaceum)Autores:Diana Alexandra Nastar Marcillo, Jimmy Nuñez, Jimmy Núñez-Pérez, José-Manuel Pais-Chanfrau, Nicolás Sebastián Pinto Mosquera, Rosario C. Espín-Valladares, Valeria Olmedo-GalarzaFuentes:googlescopusMulti-Objective Statistical Optimization of Pectinolytic Enzymes Production by an Aspergillus sp. on Dehydrated Coffee Residues in Solid-State Fermentation
ArticleAbstract: Pectinolytic enzymes are a group of enzymes widely used in the food industry. They can be obtained tPalabras claves:Aspergillus sp, coffee residues, pectinolytic enzymes, Response Surface Methodology, solid-state fermentationAutores:Brayan Santiago Chávez Arias, Jimmy Nuñez, Jimmy Núñez-Pérez, José-Manuel Pais-Chanfrau, Juan Carlos De la Vega Quintero, Santiago Zárate BacaFuentes:googlescopusMultiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris
ArticleAbstract: We have found a direct relationship between protein production in Pichia pastoris and the number ofPalabras claves:Insulin, Multiple gene copies, PICHIA PASTORIS, Protein expressionAutores:Besada-Pérez V., Cabello C., Castellanos L.R., García L., Hernández L., Hidalgo A., José-Manuel Pais-Chanfrau, Lamazares E., Mansur M., Martinez E., Plana L., Terrero Y., Valdés J., Varas L.Fuentes:googlescopusMultiple-objective optimization of lactic-fermentation parameters to obtain a functional-beverage candidate
ArticleAbstract: Background: Whey is the most abundant by-product of the cheese industry. It is estimated that it conPalabras claves:Central-composite design, Cheese industry, Fermented milk drink, Functional-beverage, Kefir grains, lactic acid bacteria, Lactic fermentation, Response Surface Methodology, Whey powder, YeastAutores:Jimmy Nuñez, Jimmy Núñez-Pérez, José-Manuel Pais-Chanfrau, Paola M. Alvarado-Cóndor, Rosario C. Espín-ValladaresFuentes:googlescopusNanotechnology applications for food and bioprocessing industries
ArticleAbstract: Nanoscience is an emerging technology today with great application in different fields. BiocatalystsPalabras claves:Bio sensors, Bioprocessing industries, nanotechnologyAutores:José-Manuel Pais-Chanfrau, Luis E. Trujillo, Luis Santiago Guerra, Rodrigo Ávalos, Silvana GrandaFuentes:googlescopusLab on a Chip: Bioreactors miniaturization for rapid optimization of biomedical processes and its impact on SARS-CoV-2 diagnosis
ReviewAbstract: Lab on a Chip (LoC) as part of Microbioreactors (MBRs) constitute an emergent technology to carry ouPalabras claves:Bioprocesses, covid-19, Micro bioreactors, Organ on a Chip, Plasmonic, Point of CareAutores:C. P. Ortega, CARLOS MARIA NOCEDA ALONSO, Carlos Noceda, Daniel A. Corredor, José-Manuel Pais-Chanfrau, Luis E. Trujillo, Michelle E. Santillán, W. S. GerFuentes:googlescopusOptimization of culture medium for large-scale production of heterologous proteins in Pichia pastoris to be used in nanoscience and other biotechnological fields
ArticleAbstract: The methylotrophic yeast Pichia pastoris became an excellent and successfully expression system forPalabras claves:Chemical defined medium, Heterologous protein expression system, PICHIA PASTORISAutores:José-Manuel Pais-Chanfrau, Luis E. TrujilloFuentes:googlescopusImproving the expression of mini-proinsulin in Pichia pastoris
ArticleAbstract: Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achiPalabras claves:AOX promoter, fed-batch culture, PICHIA PASTORIS, Recombinant human insulinAutores:Besada-Pérez V., Cabello C., Castellanos‐Serra L., Del C. Abrahantes-Pérez M., Del Toro Y., García Y., Hernández L., Hidalgo A., José-Manuel Pais-Chanfrau, Licor L., Mansur M., Martinez E., Plana L., Támbara Y., Valdés J.Fuentes:googlescopus