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Complete sucrose hydrolysis by heat-killed recombinant Pichia pastoris cells entrapped in calcium alginate
ArticleAbstract: Background: An ideal immobilized biocatalyst for the industrial-scale production of invert sugar shoPalabras claves:Alginate, Immobilization, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:Echemendia F.M., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Quintero Y., Ramírez R., Sobrino A.Fuentes:scopusAn efficient protocol for sugarcane (Saccharum spp. L.) transformation mediated by Agrobacterium tumefaciens
ArticleAbstract: This is the first successful report of the recovery of morphologically normal transgenic sugarcane pPalabras claves:Agrobacterium, SACCHARUM, TransformationAutores:Arencibia A.D., Carmona E.R., Chan M.T., Luis E. Trujillo, Oramas P., Tellez-Rodriguez P., Yu S.M.Fuentes:scopusConstitutive expression of enzymatically active Gluconacetobacter diazotrophicus levansucrase in the methylothrophic yeast Pichia pastoris
ArticleAbstract: Gluconacetobacter diazotrophicus levansucrase (LsdA) was constitutively expressed in P. pastoris undPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Glyceraldehyde-3-phosphate dehydrogenase promoter, Levansucrase, PICHIA PASTORISAutores:Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Sotolongo M., Támbara Y.Fuentes:googlescopusConstitutive high-level expression of a codon-optimized β-fructosidase gene from the hyperthermophile Thermotoga maritima in Pichia pastoris
ArticleAbstract: Enzymes for use in the sugar industry are preferred to be thermotolerant. In this study, a syntheticPalabras claves:Fructosidase, GAP promoter, Invert sugar, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:González E.M., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Pérez E.R.Fuentes:scopusAgrobacterium-mediated transformation of sugarcane (Saccharum officinarum L): A new methodology for the transformation of this species
ArticleAbstract:Palabras claves:Autores:Arencibia A.D., Blanco D., Carmona E.R., Cruz A., de la Riva G.A., Enriquez-Obregon G.A., Hernandez F., Luis E. Trujillo, Oramas P., Perez M., Prieto-Sansonov D., Selman- Houssein G., Tellez-Rodriguez P., Vazquez-Padron R.I.Fuentes:scopusFrom the laboratory to the industry: Enzymatic production and applications of shortchain fructooligosaccharides (FOS). Recent advances and current perspectives
ReviewAbstract: The production of high yields of oligosaccharides of specific chain length from simple raw materialsPalabras claves:Enzymatic production, Enzymes, oligosaccharidesAutores:L. K. Ponce, Luis E. Trujillo, Rodrigo Ávalos, Thelvia I. Ramos Gómez, Vanessa E. MarcilloFuentes:scopusFructo-oligosaccharides production by the Gluconacetobacter diazotrophicus levansucrase expressed in the methylotrophic yeast Pichia pastoris
ArticleAbstract: Levansucrase (LsdA) (EC 2.4.1.10) from Gluconacetobacter diazotrophicus (formerly Acetobacter diazotPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Levansucrase, PICHIA PASTORIS, recombinant expressionAutores:Arrieta J.G., Dafhnis F., García J., Hernandez L., Luis E. Trujillo, Perez M., Támbara Y., Valdés J.Fuentes:scopusFructooligosaccharides production by Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase constitutively expressed to high levels in Pichia pastoris
ArticleAbstract: The non-saccharolytic yeast Pichia pastoris was engineered to express constitutively the mature regiPalabras claves:1-kestose, FOS, Fructooligosaccharides, Fructosyltransferase, GAP promoter, PICHIA PASTORISAutores:Alfonso D., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Musacchio Lasa A., Pérez E.R., Pimentel E., Ramírez R., Sobrino A.Fuentes:scopusFructooligosaccharides production by immobilized Pichia pastoris cells expressing Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase
ArticleAbstract: Fructooligosaccharides (FOSs)—fructose-based oligosaccharides—are typical prebiotics with health-proPalabras claves:Calcium alginate, Cell immobilization, Fructooligosaccharides, Fructosyltransferase, PICHIA PASTORISAutores:Alfonso D., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Pimentel E., Ramírez R., Rodríguez I., Sobrino A.Fuentes:scopusLab on a Chip: Bioreactors miniaturization for rapid optimization of biomedical processes and its impact on SARS-CoV-2 diagnosis
ReviewAbstract: Lab on a Chip (LoC) as part of Microbioreactors (MBRs) constitute an emergent technology to carry ouPalabras claves:Bioprocesses, covid-19, Micro bioreactors, Organ on a Chip, Plasmonic, Point of CareAutores:C. P. Ortega, CARLOS MARIA NOCEDA ALONSO, Carlos Noceda, Daniel A. Corredor, José-Manuel Pais-Chanfrau, Luis E. Trujillo, Michelle E. Santillán, W. S. GerFuentes:googlescopus