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Constitutive expression of enzymatically active Gluconacetobacter diazotrophicus levansucrase in the methylothrophic yeast Pichia pastoris
ArticleAbstract: Gluconacetobacter diazotrophicus levansucrase (LsdA) was constitutively expressed in P. pastoris undPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Glyceraldehyde-3-phosphate dehydrogenase promoter, Levansucrase, PICHIA PASTORISAutores:Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Sotolongo M., Támbara Y.Fuentes:googlescopusFructo-oligosaccharides production by the Gluconacetobacter diazotrophicus levansucrase expressed in the methylotrophic yeast Pichia pastoris
ArticleAbstract: Levansucrase (LsdA) (EC 2.4.1.10) from Gluconacetobacter diazotrophicus (formerly Acetobacter diazotPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Levansucrase, PICHIA PASTORIS, recombinant expressionAutores:Arrieta J.G., Dafhnis F., García J., Hernandez L., Luis E. Trujillo, Perez M., Támbara Y., Valdés J.Fuentes:scopusExpression in Escherichia coli of the IpdA gene, protein sequence analysis and immunological characterization of the P64k protein from Neisseria meningitidis
ArticleAbstract: By making use of recombinant DNA technology it is possible to characterize meningococcal outer membrPalabras claves:Autores:Alvarez A., Besada-Pérez V., Caballero E., Carmenate T., Coizeau E., Estrada R., González L.J., González S., Guillén-Nieto G.E., Herrera L., Musacchio Lasa A., Nazabal C., Padrón G., Silva R., Támbara Y., Vivian Morera CórdovaFuentes:scopusOn the isolation of immunostimulatory active acemannan from Aloe barbadensis
ArticleAbstract: Acemannan from Aloe barbadensis was obtained with four processes: 1) size exclusion chromatography (Palabras claves:Acemannan, Aloe Vera, Cetyltrimethylammonium bromide, HBsAg, purificationAutores:Aguilar J., Alonso M., Cremata J.A., Eugenio Hardy, Gerwig G., Kamerling H., López M., Mayo O., Prieto E., Támbara Y.Fuentes:scopusImproving the expression of mini-proinsulin in Pichia pastoris
ArticleAbstract: Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achiPalabras claves:AOX promoter, fed-batch culture, PICHIA PASTORIS, Recombinant human insulinAutores:Besada-Pérez V., Cabello C., Castellanos‐Serra L., Del C. Abrahantes-Pérez M., Del Toro Y., García Y., Hernández L., Hidalgo A., José-Manuel Pais-Chanfrau, Licor L., Mansur M., Martinez E., Plana L., Támbara Y., Valdés J.Fuentes:googlescopus