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Constitutive expression of enzymatically active Gluconacetobacter diazotrophicus levansucrase in the methylothrophic yeast Pichia pastoris
ArticleAbstract: Gluconacetobacter diazotrophicus levansucrase (LsdA) was constitutively expressed in P. pastoris undPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Glyceraldehyde-3-phosphate dehydrogenase promoter, Levansucrase, PICHIA PASTORISAutores:Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Sotolongo M., Támbara Y.Fuentes:googlescopusFructo-oligosaccharides production by the Gluconacetobacter diazotrophicus levansucrase expressed in the methylotrophic yeast Pichia pastoris
ArticleAbstract: Levansucrase (LsdA) (EC 2.4.1.10) from Gluconacetobacter diazotrophicus (formerly Acetobacter diazotPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Levansucrase, PICHIA PASTORIS, recombinant expressionAutores:Arrieta J.G., Dafhnis F., García J., Hernandez L., Luis E. Trujillo, Perez M., Támbara Y., Valdés J.Fuentes:scopusIdentification and recombinant expression of a bacterial exolevanase useful for the production of high fructose syrups
ArticleAbstract: The sugarcane endophyte Gluconacetobacter diazotrophicus produces levan from sucrose by a secreted lPalabras claves:Autores:Alfonso D., Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Menéndez C., Ramírez R.Fuentes:googlescopusRecombinant expression of active Penicillium minioluteum endodextranase in alternative hosts
ArticleAbstract: The dextranase gene from the fungus Penicillium minioluteum was expressed in Escherichia coli. The rPalabras claves:Cell bombardment, Dextranase, ESCHERICHIA coli, Penicillium minioluteum, recombinant expressionAutores:Arrieta J.G., Dafhnis F., Fuentes A.D., Garcia B.M., Hernandez L., Luis E. Trujillo, Quintero O., Ramos P.L.Fuentes:scopusProduction of highly polymerized bacterial levan in two eukaryotic hosts of biotechnological interest
ArticleAbstract: Bacterial levan [β(2,6)-linked polyfructan] has potential applications in the food, bio-energetic, mPalabras claves:Catalytical properties, Fructan, Gluconacetobacter diazotrophicus, Levan, Levansucrase, N-glycosilation, PICHIA PASTORIS, Transgenic tobaccoAutores:Arrieta J.G., Banguela A., Borroto C., Enrique Pérez C.M., Hernandez L., Luis E. Trujillo, Pujol M., Ramírez R., Rodríguez R.Fuentes:scopus