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A sandwich-type enzyme-linked immunosorbent assay for the analysis of recombinant human interferon α-2b
ArticleAbstract: The ability of a previously developed sandwich-type enzyme-linked immunosorbent assay (ELISA) for diPalabras claves:Elisa, ESCHERICHIA coli, folding, IFN-alpha, monoclonal antibodiesAutores:Espino Y., Eugenio Hardy, Franco A., Furrazola G., Santana-Milián H.Fuentes:scopusElution of lipopolysaccharides from polyacrylamide gels
ArticleAbstract: A simple procedure for elution in water of bacterial lipopolysaccharides (LPS) from sodium dodecyl sPalabras claves:Elution, Gel electrophoresis, LAL, Silver staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio Hardy, Guerra M., Pupo E., Santana-Milián H.Fuentes:scopusHigh yield elution of proteins from sodium dodecyl sulfate- polyacrylamide gels at the low-picomole level. Application to N-terminal sequencing of a scarce protein and to in-solution biological activity analysis of on-gel renatured proteins
Conference ObjectAbstract: A simple, reliable procedure for practically quantitative (90-98%) and fast (<30 min) elution of proPalabras claves:Passive elution, Protein detection, Protein micropurification, SDS PAGE, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V., Santana-Milián H.Fuentes:scopusMice immunization with gel electrophoresis-micropurified bacterial lipopolysaccharides
ArticleAbstract: Some evidence on the possible use of sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDSPalabras claves:Elution, Gel electrophoresis, Immunization, lipopolysaccharides, Zinc stainingAutores:Aguila A., Castellanos‐Serra L., Eugenio Hardy, Núñez J.F., Pupo E., Santana-Milián H.Fuentes:scopusMolecular characterization of recombinant human interferon alpha-2b produced in Cuba
ArticleAbstract: The recombinant human interferon α 2b (IFN-γ(2b)) produced by Cuban technology is obtained from EschPalabras claves:IFN, Molecular characterization, Recombinant human interferon alfa 2bAutores:Beldarrain A., Besada-Pérez V., Betancourt L., Brito R., Curras T., Eugenio Hardy, Ferrero J., Gil M., González L.J., Herrera L., Huerta V., Martinez E., Moya G., Pujols V., Sanchez J.C., Santana-Milián H., Sosa R., Vivian Morera CórdovaFuentes:googlescopusPharmacokinetic and pharmacodynamic characterization of a novel formulation containing co-formulated interferons alpha-2b and gamma in healthy male volunteers
ArticleAbstract: Background: More potent antitumor activity is desired in Interferon (IFN)-treated cancer patients. TPalabras claves:2'-5' oligoadenylate synthetase, Beta2-microglobulin, interferons, Neopterin, pharmacodynamics, pharmacokineticsAutores:Bello-Rivero I., Campos-Mojena R., Cruz-Ramírez A., Díaz-Machado A., García-Vega Y., González-Delgado C.A., Hernández-González I., I. Howland-Álvarez, López-Saura P.A., Martín-Trujillo A., Pérez-Rodríguez S., Santana-Milián H., Tuero-Iglesias Á.D., Valenzuela-Silva C.M.Fuentes:scopusRecovery of biologically active proteins detected with imidazole-sodium dodecyl sulfate-zinc (reverse stain) on sodium dodecyl sulfate gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Hernandez L., Santana-Milián H., Sosa A.E.Fuentes:scopus