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Analytical Biochemistry(1)
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Electrophoresis(1)
Journal of Protein Chemistry(1)
Protein Engineering, Design and Selection(1)
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scopus(5)
A procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
ArticleAbstract: We developed a technique that allows rapid protein elution from polyacrylamide gel bands at room temPalabras claves:Passive elution, Protein detection, Protein micropurification, Sodium dodecyl, Sulfate-polyacrylamide gel electrophoresis, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V.Fuentes:scopusNew tools in proteomics: A high sequence coverage by protein digestion in polyacrylamide IEF-CA, IEF-IPG and SDS gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Huerta V., Javier González L.Fuentes:scopusHigh yield elution of proteins from sodium dodecyl sulfate- polyacrylamide gels at the low-picomole level. Application to N-terminal sequencing of a scarce protein and to in-solution biological activity analysis of on-gel renatured proteins
Conference ObjectAbstract: A simple, reliable procedure for practically quantitative (90-98%) and fast (<30 min) elution of proPalabras claves:Passive elution, Protein detection, Protein micropurification, SDS PAGE, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V., Santana-Milián H.Fuentes:scopusValidation of a mutant of the pore-forming toxin sticholysin-I for the construction of proteinase-activated immunotoxins
ArticleAbstract: The use of pore-forming toxins from sea anemones (actinoporins) in the construction of immunotoxinsPalabras claves:Actinoporin, immunotoxin, Pore-forming toxin, sea anemone cytolysin, sticholysinsAutores:Álvarez C., Calvo L., Campos J., Castellanos‐Serra L., Cilli E.M., Díaz I., Fando R., Lanio M.E., Pazos I., Pentón D., Pérez-Barzaga V., Pons T., Reytor M.L., Tejuca M., Vivian Morera CórdovaFuentes:scopusRecovery of biologically active proteins detected with imidazole-sodium dodecyl sulfate-zinc (reverse stain) on sodium dodecyl sulfate gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Hernandez L., Santana-Milián H., Sosa A.E.Fuentes:scopus