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Analytical Biochemistry(2)
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ELECTROPHORESIS(1)
Electrophoresis(1)
Journal of Protein Chemistry(1)
A procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
ArticleAbstract: We developed a technique that allows rapid protein elution from polyacrylamide gel bands at room temPalabras claves:Passive elution, Protein detection, Protein micropurification, Sodium dodecyl, Sulfate-polyacrylamide gel electrophoresis, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V.Fuentes:scopusHigh yield elution of proteins from sodium dodecyl sulfate- polyacrylamide gels at the low-picomole level. Application to N-terminal sequencing of a scarce protein and to in-solution biological activity analysis of on-gel renatured proteins
Conference ObjectAbstract: A simple, reliable procedure for practically quantitative (90-98%) and fast (<30 min) elution of proPalabras claves:Passive elution, Protein detection, Protein micropurification, SDS PAGE, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V., Santana-Milián H.Fuentes:scopusSelective precipitation of zinc imidazolate: Application to the detection of biomolecules (proteins, nucleic acids, and bacterial glycolipids) separated in polyacrylamide gels
ArticleAbstract: At the start of the past decade a group of scientists from the Center for Genetic Engineering and BiPalabras claves:Electrophoresis, lipopolysaccharides, Negative staining, Nucleic acids, Polyacrylamide, proteinsAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusSelectivity of bacterial proteome fractionation based on differential solubility: A mass spectrometry evaluation
ArticleAbstract: We investigate the selectivity achieved after differential solubilization of bacterial proteomes folPalabras claves:Differential solubilization, Proteome fractionation, proteomicsAutores:Alexey Llópiz-Arzuaga, Castellanos‐Serra L., García Y., Ramos Y.Fuentes:googlescopusSingle‐step electrotransfer of reverse‐stained proteins from sodium dodecyl sulfate‐polyacrylamide gel onto reversed‐phase minicartridge and subsequent desalting and elution with a conventional high‐performance liquid chromatography gradient system for analysis
ArticleAbstract: Isolation of proteins from polyacrylamide electrophoresis gels by a novel combination of techniquesPalabras claves:High‐performance liquid chromatography, Protein microanalysis, Reverse staining, Sodium dodecyl sulfate‐polyacrylamide gel electrophoresisAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Frank R., Madrazo J., Mendez E.Fuentes:scopusRecovery of biologically active proteins detected with imidazole-sodium dodecyl sulfate-zinc (reverse stain) on sodium dodecyl sulfate gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Hernandez L., Santana-Milián H., Sosa A.E.Fuentes:scopus