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A procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
ArticleAbstract: We developed a technique that allows rapid protein elution from polyacrylamide gel bands at room temPalabras claves:Passive elution, Protein detection, Protein micropurification, Sodium dodecyl, Sulfate-polyacrylamide gel electrophoresis, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V.Fuentes:scopusExpression and folding of an interleukin-2-proinsulin fusion protein and its conversion into insulin by a single step enzymatic removal of the C-peptide and the N-terminal fused sequence
ArticleAbstract: We report the expression in E. coli of a proinsulin fusion protein carrying a modified interleukin-2Palabras claves:Enzymatic processing, Proinsulin folding, Recombinant insulinAutores:Besada-Pérez V., Castellanos‐Serra L., Eugenio Hardy, Falcón-Cama V., Fernandez C., Gonzalez M., Herrera L., Perez G., Santiago Nelson Vispo, Santos-Savio A., Silva A., Ubieta R.Fuentes:scopusNegative staining with zinc-imidazole of gel electrophoresis-separated nucleic acids
ArticleAbstract: Zinc and imidazole salts were applied for the detection of nucleic acids on either polyacrylamide orPalabras claves:Agarose gel electrophoresis, Detection of nucleic acids, Negative staining, Zinc staining, Zinc-imidazole detectionAutores:Casalvilla R., Castellanos‐Serra L., Eugenio Hardy, López E., Luis E. Trujillo, Pupo E., Sosa A.E.Fuentes:scopusRecovery of biologically active proteins detected with imidazole-sodium dodecyl sulfate-zinc (reverse stain) on sodium dodecyl sulfate gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Hernandez L., Santana-Milián H., Sosa A.E.Fuentes:scopus