Mostrando 10 resultados de: 17
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Enzyme and Microbial Technology(9)
Biotechnology and Bioengineering(2)
Annals of the New York Academy of Sciences(1)
Biocatalysis and Biotransformation(1)
Biotechnology and Applied Biochemistry(1)
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Bioquímica(7)
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scopus(17)
A criterion for the selection of monophasic solvents for enzymatic synthesis
ArticleAbstract: Penicillin acylase can be used for the production of β-lactamic antibiotics which have a wide rangePalabras claves:Antibiotic, Enzyme stability, Equilibrium-controlled synthesis, Organic solvent selection, Penicillin acylaseAutores:Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M., Terreni M.Fuentes:scopusAdditional stabilization of penicillin G acylase-agarose derivatives by controlled chemical modification with formaldehyde
ArticleAbstract: We have tested the effect of chemical modifications with formaldehyde on the activity/stability of iPalabras claves:chemical modification of proteins, Enzyme stabilization, formaldehyde treatment of enzymes, immobilization-stabilization of enzymesAutores:Alvaro G., Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M.Fuentes:scopusDynamic reaction design of enzymic biotransformations in organic media: Equilibrium-controlled synthesis of antibiotics by penicillin G acylase
ArticleAbstract: Parameters relevant to the thermodynamically controlled synthesis of cephalothin utilizing highly acPalabras claves:Autores:Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M.Fuentes:scopusFacile synthesis of artificial enzyme nano-environments via solid-phase chemistry of immobilized derivatives: Dramatic stabilization of penicillin acylase versus organic solvents
ArticleAbstract: A method to generate a highly hydrophilic nano-environment surrounding immobilized enzymes [penicillPalabras claves:Artificial nano-environments, Dextrans, Enzymes in organic media, Immobilized enzymes, Solid-phase modification of proteins, Stabilization of enzymesAutores:Caanan-Haden L., Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M., Rodes L.Fuentes:scopusEnzyme reaction engineering: Synthesis of antibiotics catalysed by stabilized penicillin G acylase in the presence of organic cosolvents
ArticleAbstract: By using very active and very stable penicillin G acylase (PGA)-agarose derivatives we have studiedPalabras claves:antibiotic synthesis by penicillin G acylase, design of thermodynamically controlled synthesis, enzymes in organic solvents, Enzymes in organic synthesisAutores:Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M.Fuentes:scopusImmobilization‐stabilization of α‐chymotrypsin by covalent attachment to aldehyde‐agarose gels
ArticleAbstract: We have developed a strategy for immobilization‐stabilization of α‐chymotrypsin by multipoint covalePalabras claves:effect of denaturing agents, enzyme‐support multipoint attachment, stabilization of chymotrypsinAutores:Bastida A., Cristina M. Rosell, Cuesta C., Fernandez‐Lafuente R., Guisan J.M.Fuentes:scopusStabilization of heterodimeric enzyme by multipoint covalent immobilization: Penicillin G acylase from Kluyvera citrophila
ArticleAbstract: We have developed a strategy for immobilization–stabilization of penicillin G acylase (PGA) from KluPalabras claves:immobilization–stabilization of penicillin G acylase, Kluyvera citrophila, Penicillin G acylase, reactivation of enzyme derivatives, stabilization of multimeric enzymesAutores:Alvaro G., Cristina M. Rosell, Fernandez‐Lafuente R., Garcia-Lopez J.L., Guisan J.M., Tagliani A.Fuentes:scopusStabilization of immobilized enzymes against organic solvents: Complete hydrophylization of enzymes environments by solidphase chemistry with poly-functional macromolecules.
Book PartAbstract:Palabras claves:Autores:Caanan-Haden L., Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M., Rodes L.Fuentes:scopusStrategies for enzyme stabilization by intramolecular crosslinking with bifunctional reagents
ArticleAbstract: A original strategy to obtain intramolecular crosslinking is discussed. This strategy consisted of tPalabras claves:chemical modification of proteins, Formaldehyde, GLUTARALDEHYDE, Penicillin G acylase, Protein crosslinking, Stabilization of enzymesAutores:Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M., Rodríguez V.Fuentes:scopusSyntheses of pharmaceutical oligosaccharides catalyzed by immobilized-stabilized derivatives of different β-galactosidases
ArticleAbstract: We have developed strategies for immobilizing and stabilizing enzymes with very different and complePalabras claves:Biocatalysis, enzyme immobilization-stabilization, Enzymes, oligosaccharides, Organic synthesis, regioselectivity, β-galactosidasesAutores:Bastida A., Cristina M. Rosell, Fernández-Mayoralas A., Fernandez‐Lafuente R., Guisan J.M., Loṕez R., Martín-Lomas M., Rodríguez V., Santana C., Soler G.Fuentes:scopus