Mostrando 10 resultados de: 22
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Enzyme and Microbial Technology(9)
Annals of the New York Academy of Sciences(2)
Biotechnology and Bioengineering(2)
Journal of Molecular Catalysis(2)
Biocatalysis and Biotransformation(1)
A criterion for the selection of monophasic solvents for enzymatic synthesis
ArticleAbstract: Penicillin acylase can be used for the production of β-lactamic antibiotics which have a wide rangePalabras claves:Antibiotic, Enzyme stability, Equilibrium-controlled synthesis, Organic solvent selection, Penicillin acylaseAutores:Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M., Terreni M.Fuentes:scopusAdditional stabilization of penicillin G acylase-agarose derivatives by controlled chemical modification with formaldehyde
ArticleAbstract: We have tested the effect of chemical modifications with formaldehyde on the activity/stability of iPalabras claves:chemical modification of proteins, Enzyme stabilization, formaldehyde treatment of enzymes, immobilization-stabilization of enzymesAutores:Alvaro G., Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M.Fuentes:scopusDesign of novel biocatalysts by 'bioimprinting' during unfolding-refolding of fully dispersed covalently immobilized enzymes
Conference ObjectAbstract:Palabras claves:Autores:Blanco R.M., Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M., Soler G.Fuentes:scopusFacile synthesis of artificial enzyme nano-environments via solid-phase chemistry of immobilized derivatives: Dramatic stabilization of penicillin acylase versus organic solvents
ArticleAbstract: A method to generate a highly hydrophilic nano-environment surrounding immobilized enzymes [penicillPalabras claves:Artificial nano-environments, Dextrans, Enzymes in organic media, Immobilized enzymes, Solid-phase modification of proteins, Stabilization of enzymesAutores:Caanan-Haden L., Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M., Rodes L.Fuentes:scopusEnzyme reaction engineering: Synthesis of antibiotics catalysed by stabilized penicillin G acylase in the presence of organic cosolvents
ArticleAbstract: By using very active and very stable penicillin G acylase (PGA)-agarose derivatives we have studiedPalabras claves:antibiotic synthesis by penicillin G acylase, design of thermodynamically controlled synthesis, enzymes in organic solvents, Enzymes in organic synthesisAutores:Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M.Fuentes:scopusDynamic reaction design of enzymic biotransformations in organic media: Equilibrium-controlled synthesis of antibiotics by penicillin G acylase
ArticleAbstract: Parameters relevant to the thermodynamically controlled synthesis of cephalothin utilizing highly acPalabras claves:Autores:Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M.Fuentes:scopusImmobilization-stabilization of proteases as a tool to improve the industrial design of peptide synthesis
Conference ObjectAbstract: Synthesis of dipeptides benzoyl arginine leucinamide and kyotorphin catalyzed by highly stabilized dPalabras claves:Autores:Alvaro G., Bastida A., Blanco R.M., Cristina M. Rosell, Cuesta C., Fernandez‐Lafuente R., Guisan J.M.Fuentes:scopusImmobilization‐stabilization of α‐chymotrypsin by covalent attachment to aldehyde‐agarose gels
ArticleAbstract: We have developed a strategy for immobilization‐stabilization of α‐chymotrypsin by multipoint covalePalabras claves:effect of denaturing agents, enzyme‐support multipoint attachment, stabilization of chymotrypsinAutores:Bastida A., Cristina M. Rosell, Cuesta C., Fernandez‐Lafuente R., Guisan J.M.Fuentes:scopusModification of enzyme properties by the use of inhibitors during their stabilisation by multipoint covalent attachment
ArticleAbstract: The effect of a number of inhibitors adsorbed at the active site of penicillin acylase during immobiPalabras claves:inhibitor, Penicillin acylase, StabilisationAutores:Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M.Fuentes:scopusModulation of the properties of penicillin G acylase by acyl donor substrates during N-protection of amino compounds
ArticleAbstract: The rates and yields in the synthesis of N-protected amino compounds catalyzed by penicillin G acylaPalabras claves:Enzyme modulation, Enzymes (in fine chemistry), Optically pure compound synthesis, Penicillin G acylase, Protection of amino compoundsAutores:Cristina M. Rosell, Fernandez‐Lafuente R., Guisan J.M.Fuentes:scopus