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Complete sucrose hydrolysis by heat-killed recombinant Pichia pastoris cells entrapped in calcium alginate
ArticleAbstract: Background: An ideal immobilized biocatalyst for the industrial-scale production of invert sugar shoPalabras claves:Alginate, Immobilization, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:Echemendia F.M., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Quintero Y., Ramírez R., Sobrino A.Fuentes:scopusConstitutive expression of enzymatically active Gluconacetobacter diazotrophicus levansucrase in the methylothrophic yeast Pichia pastoris
ArticleAbstract: Gluconacetobacter diazotrophicus levansucrase (LsdA) was constitutively expressed in P. pastoris undPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Glyceraldehyde-3-phosphate dehydrogenase promoter, Levansucrase, PICHIA PASTORISAutores:Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Sotolongo M., Támbara Y.Fuentes:googlescopusConstitutive high-level expression of a codon-optimized β-fructosidase gene from the hyperthermophile Thermotoga maritima in Pichia pastoris
ArticleAbstract: Enzymes for use in the sugar industry are preferred to be thermotolerant. In this study, a syntheticPalabras claves:Fructosidase, GAP promoter, Invert sugar, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:González E.M., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Pérez E.R.Fuentes:scopusFructo-oligosaccharides production by the Gluconacetobacter diazotrophicus levansucrase expressed in the methylotrophic yeast Pichia pastoris
ArticleAbstract: Levansucrase (LsdA) (EC 2.4.1.10) from Gluconacetobacter diazotrophicus (formerly Acetobacter diazotPalabras claves:1-kestose, Fructo-oligosaccharides, Gluconacetobacter diazotrophicus, Levansucrase, PICHIA PASTORIS, recombinant expressionAutores:Arrieta J.G., Dafhnis F., García J., Hernandez L., Luis E. Trujillo, Perez M., Támbara Y., Valdés J.Fuentes:scopusFructooligosaccharides production by Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase constitutively expressed to high levels in Pichia pastoris
ArticleAbstract: The non-saccharolytic yeast Pichia pastoris was engineered to express constitutively the mature regiPalabras claves:1-kestose, FOS, Fructooligosaccharides, Fructosyltransferase, GAP promoter, PICHIA PASTORISAutores:Alfonso D., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Musacchio Lasa A., Pérez E.R., Pimentel E., Ramírez R., Sobrino A.Fuentes:scopusFructooligosaccharides production by immobilized Pichia pastoris cells expressing Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase
ArticleAbstract: Fructooligosaccharides (FOSs)—fructose-based oligosaccharides—are typical prebiotics with health-proPalabras claves:Calcium alginate, Cell immobilization, Fructooligosaccharides, Fructosyltransferase, PICHIA PASTORISAutores:Alfonso D., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Pimentel E., Ramírez R., Rodríguez I., Sobrino A.Fuentes:scopusFunctional production and secretion of the Gluconacetobacter diazotrophicus fructose-releasing exo-levanase (LsdB) in Pichia pastoris
ArticleAbstract: The gene encoding the fructose-releasing exo-levanase (LsdB; EC 3.2.1.65) from Gluconacetobacter diaPalabras claves:Alcohol oxidase, AOX, AOX1 promoter, Gap, GAP promoter, Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus levanase, Gluconacetobacter diazotrophicus levansucrase, Glyceraldehyde 3-phosphate dehydrogenase, Levanase, LsdA, LsdB, PICHIA PASTORISAutores:Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Menéndez C.Fuentes:googlescopusIdentification and recombinant expression of a bacterial exolevanase useful for the production of high fructose syrups
ArticleAbstract: The sugarcane endophyte Gluconacetobacter diazotrophicus produces levan from sucrose by a secreted lPalabras claves:Autores:Alfonso D., Arrieta J.G., Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Luis E. Trujillo, Menéndez C., Ramírez R.Fuentes:googlescopusScaling-up batch conditions for efficient sucrose hydrolysis catalyzed by an immobilized recombinant Pichia pastoris cells in a stirrer tank reactor
ArticleAbstract: Background: Invert sugar is used greatly in food and pharmaceutical industries. This paper describesPalabras claves:Bioreactor, Calcium alginate beads, Enzymatic biocatalyst, Immobilized biocatalyst, Industrial biotechnology, Invert sugar, Invertase, Kinetic model, Scale-up, Stirred tank reactor, Sucrose inversionAutores:Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Rodríguez I., Sobrino A.Fuentes:scopusRecombinant expression of active Penicillium minioluteum endodextranase in alternative hosts
ArticleAbstract: The dextranase gene from the fungus Penicillium minioluteum was expressed in Escherichia coli. The rPalabras claves:Cell bombardment, Dextranase, ESCHERICHIA coli, Penicillium minioluteum, recombinant expressionAutores:Arrieta J.G., Dafhnis F., Fuentes A.D., Garcia B.M., Hernandez L., Luis E. Trujillo, Quintero O., Ramos P.L.Fuentes:scopus