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Journal of Biotechnology(2)
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A type II protein secretory pathway required for levansucrase secretion by Gluconacetobacter diazotrophicus
ArticleAbstract: The endophytic diazotroph Gluconacetobacter diazotrophicus secretes a constitutively expressed levanPalabras claves:Autores:Alfonso D., Arrieta J.G., Hernandez L., Luis E. Trujillo, Menéndez C., Ramírez R., Soto M., Sotolongo M.Fuentes:scopusDevelopment of soluble and immobilized Biocatalysts based on a recombinant thermostable β-fructosidase enabling complete sucrose inversion at pasteurization temperatures
ArticleAbstract: Biocatalysts for the industrial production of invert sugar are preferred to stably operate at high sPalabras claves:Immobilization, Invert syrup, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:Basabe L., Cutiño-Ávila B.V., Del Monte-Martínez A., Hernandez L., Luis E. Trujillo, Martínez D., Menéndez C., Pérez E.R., Ramírez R., Sobrino A.Fuentes:scopusConstitutive high-level expression of a codon-optimized β-fructosidase gene from the hyperthermophile Thermotoga maritima in Pichia pastoris
ArticleAbstract: Enzymes for use in the sugar industry are preferred to be thermotolerant. In this study, a syntheticPalabras claves:Fructosidase, GAP promoter, Invert sugar, Invertase, PICHIA PASTORIS, Thermotoga maritimaAutores:González E.M., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Pérez E.R.Fuentes:scopusFructooligosaccharides production by Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase constitutively expressed to high levels in Pichia pastoris
ArticleAbstract: The non-saccharolytic yeast Pichia pastoris was engineered to express constitutively the mature regiPalabras claves:1-kestose, FOS, Fructooligosaccharides, Fructosyltransferase, GAP promoter, PICHIA PASTORISAutores:Alfonso D., Hernandez L., Luis E. Trujillo, Martínez D., Mazola Y., Menéndez C., Musacchio Lasa A., Pérez E.R., Pimentel E., Ramírez R., Sobrino A.Fuentes:scopusFunctional production and secretion of the Gluconacetobacter diazotrophicus fructose-releasing exo-levanase (LsdB) in Pichia pastoris
ArticleAbstract: The gene encoding the fructose-releasing exo-levanase (LsdB; EC 3.2.1.65) from Gluconacetobacter diaPalabras claves:Alcohol oxidase, AOX, AOX1 promoter, Gap, GAP promoter, Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus levanase, Gluconacetobacter diazotrophicus levansucrase, Glyceraldehyde 3-phosphate dehydrogenase, Levanase, LsdA, LsdB, PICHIA PASTORISAutores:Banguela A., Hernandez L., José-Manuel Pais-Chanfrau, Menéndez C.Fuentes:googlescopusHigh levan accumulation in transgenic tobacco plants expressing the Gluconacetobacter diazotrophicus levansucrase gene
ArticleAbstract: Bacterial levansucrase (EC 2.4.1.10) converts sucrose into non-linear levan consisting of long β(2,6Palabras claves:FOS, Fructan, Gluconacetobacter diazotrophicus, Levan, Levansucrase, Transgenic tobaccoAutores:Arrieta J.G., Banguela A., Hernandez L., Luis E. Trujillo, Menéndez C., Rodríguez R.Fuentes:scopusRecombinant expression of active Penicillium minioluteum endodextranase in alternative hosts
ArticleAbstract: The dextranase gene from the fungus Penicillium minioluteum was expressed in Escherichia coli. The rPalabras claves:Cell bombardment, Dextranase, ESCHERICHIA coli, Penicillium minioluteum, recombinant expressionAutores:Arrieta J.G., Dafhnis F., Fuentes A.D., Garcia B.M., Hernandez L., Luis E. Trujillo, Quintero O., Ramos P.L.Fuentes:scopusRecovery of biologically active proteins detected with imidazole-sodium dodecyl sulfate-zinc (reverse stain) on sodium dodecyl sulfate gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Hernandez L., Santana-Milián H., Sosa A.E.Fuentes:scopusSodERF3, a novel sugarcane ethylene responsive factor (ERF), enhances salt and drought tolerance when overexpressed in tobacco plants
ArticleAbstract: The molecular signals and pathways that govern biotic and abiotic stress responses in sugarcane arePalabras claves:ABA, abiotic stress, EAR motif, Ethylene responsive factors, Salt and drought tolerance, SugarcaneAutores:Borras-Hidalgo O., Coll Y., Hernández I., Hernandez L., Luis E. Trujillo, Menéndez C., Ochogavía M.E., Sotolongo M., Thomma B.P.H.J., Vera P.Fuentes:scopus