Determination of Juglone concentration for differentiate two Musa spp cultivars

Abstract:

The toxins production by fungal pathogenic can enhance penetration and colonization of the host tissues and reproduce symptoms of diseases. Juglone is the main and the most toxic metabolite characterized from the culture filtrate of Mycosphaerella fijiensis. In the present work it was used different concentrations of juglone (10, 25, 50 and 100 mg.l-1) and a control solution of methanol 10%. Foliar injections were performed in the underside of two last open leaves of Grande naine (AAA) and Fougamou (ABB) cultivars. The percentage of necrosis per plant was evaluated at 24 hours after the injection. The concentration of 100 mg.l-1 was able to produce necrotic symptoms in both cultivars; nevertheless with the 50 mg.l-1 was possible to obtain differences between the two cultivars. This result established a concentration for screening two Musa spp cultivars with different resistance levels to Black Leaf Streak by the use of juglone.Key word: banana leaf streak, Mycosphaerella fijiensis, selection, toxins

Año de publicación:

2002

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