Effect of laser phototherapy on the release of TNF-α and MMP-1 by endodontic sealer-stimulated macrophages

Abstract:

Objective: Our aim was to analyze the effect of laser phototherapy on the secretory activity of macrophages activated by interferon-γ (IFN-γ) and lipopolysaccharide (LPS), and stimulated by substances leached from an epoxy resin-based sealer (AH-Plus) and a calcium hydroxide-based sealer (Sealapex). Background Data: Laser phototherapy can modulate the inflammatory process, improving wound healing. This type of therapy could be useful for modulating postoperative symptoms seen after endodontic treatment. Materials and Methods: Cytotoxicity was indirectly assessed by measuring mitochondrial activity. Macrophages were stimulated by the leached substances or not (controls), and the groups were then irradiated or not. The secretion of pro-inflammatory cytokines (TNF-α and MMP-1) was analyzed using ELISA. Two irradiations at 6-h intervals were done with an As-Ga-Al diode laser (780 nm, 70 mW, spot size 4.0 mm2, 3 J/cm2, for 1.5 sec) in contact mode. Results: The sealers were non-cytotoxic to macrophages. The production of TNF-α was significantly decreased by laser phototherapy, regardless of experimental group. The level of secretion of MMP-1 was similar in all groups. Conclusion: Based on the conditions of this study we concluded that in activated macrophages, laser phototherapy impairs the secretion of the pro-inflammatory cytokine TNF-α, but has no influence on MMP-1 secretion. © 2009 Mary Ann Liebert, Inc.

Año de publicación:

2009

Keywords:

Fuente:

scopus