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Effect of low-power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts

Abstract:

Background and Objectives: In dentistry, low-power lasers have been used in the treatment of dentin hypersensitivity, gingivitis, periodontitis, and different forms of oral ulcers. This in vitro study focuses on the biostimulation of NIH-3T3 fibroblasts by a low-power Ga-As-pulsed laser. Study Design/Materials and Methods: We have studied cell growth and procollagen synthesis of cultured fibroblasts submitted to low-power laser irradiation with energy densities varying from 3 to 5 J/cm2 over a period of 1-6 days. The light source was a 120 mW Ga-As diode laser (λ = 904 nm). Growth curves and procollagen immunoprecipitation were obtained. Results: Irradiation of 3 and 4 J/cm2 increased the cell numbers about threefold to sixfold comparing to control cultures. However, this effect was restricted to a small range of energy densities since 5 J/cm2 had no effect on cell growth. The energy density of 3 J/cm2 remarkably increased cell growth, with no effect on procollagen synthesis, as demonstrated by the immunoprecipitation analysis. Conclusions: Our results showed that a particular laser irradiation stimulates fibroblast proliferation, without impairing procollagen synthesis. © 2002 Wiley-Liss, Inc.