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Elaboration of a bioprepared with probiotic effect from a mixed culture of lactic bacteria and yeasts

Abstract:

The use of bacteria as beneficial biological agents being used as food ingredients or as active components of food supplements dates back to the early 1900s. The objective of this study was elaboration a bioprepared whit probiotic effect from a mixed culture of lactic bacteria and yeasts. The bioprepared substrate contained 57.5% orange vinasse and 30% Sugar cane molasses. The strains L acidophillus, L bulgaricus, S thermophillus, Kluyveromyces fragilis L-4uclv and Saccharomyces cerevisiae, it was grown in sterilized milk. Were in oculated 12.5% in the substratum molasses-vinasse. Finally incubated during 24h at 37.C. The results of the biopreparation was 18% Dry matter, 3.3% ash, 19% Crude protein, 12% True protein and 3.2% Ether extract. The microorganism counts were 9x109 cfu/mL, 0.75% organic acids and 95% viability was. Color was, in the CIELab∗System: L∗31.85, a∗11.48, b∗24.52, C∗27.08 and H64.91, similar to the HTML code #61382B. pH initial was 4.4 and after 72h it was established in 3.86 the value maintained for 90 d, at a temperature 12 ±2.C. There were no differences in physical characteristics. The parameters bromatology and microbiology there was difference (P<0.05). The results showed that by-products as vinasse and molasses are good and economic substrates feed for probiotic grow and obtain an acceptable probiotic for animal feed.