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Elaboration of a bioprepared with probiotic effect from a mixed culture of lactic bacteria and yeasts

Abstract:

The use of bacteria as beneficial biological agents being used as food ingbkp_redients or as active components of food supplements dates back to the early 1900s. The objective of this study was elaboration a bioprepared whit probiotic effect from a mixed culture of lactic bacteria and yeasts. The bioprepared substrate contained 57.5% orange vinasse and 30% Sugar cane molasses. The strains L acidophillus, L bulgaricus, S thermophillus, Kluyveromyces fragilis L-4uclv and Saccharomyces cerevisiae, it was grown in sterilized milk. Were in oculated 12.5% in the substratum molasses-vinasse. Finally incubated during 24h at 37.C. The results of the biopreparation was 18% Dry matter, 3.3% ash, 19% Crude protein, 12% True protein and 3.2% Ether extract. The microorganism counts were 9x109 cfu/mL, 0.75% organic acids and 95% viability was. Color was, in the CIELab∗System: L∗31.85, a∗11.48, b∗24.52, C∗27.08 and H64.91, similar to the HTML code #61382B. pH initial was 4.4 and after 72h it was established in 3.86 the value maintained for 90 d, at a temperature 12 ±2.C. There were no differences in physical characteristics. The parameters bromatology and microbiology there was difference (P<0.05). The results showed that by-products as vinasse and molasses are good and economic substrates feed for probiotic grow and obtain an acceptable probiotic for animal feed.