Morphological and molecular identification of Fusarium species associated with vascular wilt of babaco (Vasconcellea heilbornii var. pentagona Badillo)

Abstract:

Pathogenic isolates of Fusarium spp. associated with babaco (Vasconcellea heilbornii var. pentagona Badillo) were characterized morphologically and molecularly. Babaco roots with symptoms of root rot were collected in major areas in southern Ecuador, and monosporic isolates were obtained. Twenty-one isolates associated with the vascular wilt of babaco (VWB) were characterized culturally, morphologicaly, and molecularly. Amplification of ITS (Internal Transcribed Spacer) subregions placed between the ribosomal genes (rDNA) 18S was performed using specific primers for Fusarium identification. Based on morphological and cultural characteristics, 12 isolates were identified as belonging to Fusarium. Analysis of nucleotide sequences confirmed that all of them belonged to the Fusarium genus: Fusarium oxysporum (Schlechtendahl emend. Snyder & Hansen)(nine isolates), Fusarium camptoceras (Wollenweber & Reinking emend. Marasas & Logrieco)(one isolate), Fusarium temperatum (Scaufl. & Munaut)(one isolate), and Fusarium sp.(one isolate). This is the first report of these four Fusarium species causing VWB in the south of Ecuador.

Año de publicación:

2016

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