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Pilot study to develop a food composition database for a Southern region of Ecuador

Abstract:

To document and warrant the sample traceability and data quality, preanalytical and post-analytical protocols were prepared based on recommendations of LATINFOODS and EUROfir. A sampling plan was designed to collect representative samples from the main local markets and supermarkets. Considering that variability data for the selected key nutrients in those foods is not available, a sample size of n= 12 was taken (pilot study) and mixed as composite sample. One analytical portion was analyzed in triplicate determining: moisture and dry matter (AOAC, 2002; Anklam et al., 2001), ash (AOAC, 2002; Sullivan and Carpenter, 1993), total fat by Weibull (AOAC, 2002; Sullivan and Carpenter, 1993), total nitrogen by Kjeldahl (AOAC, 2002; Sullivan and Carpenter, 1993) and total and available carbohydrates (by difference).