Quinoa (Chenopodium quinoa Willd.) proteins as source of antioxidant and antiproliferative peptides after in vitro gastrointestinal digestion

Abstract:

Quinoa is a pseudocereal traditionally consumed by Andean cultures that is attracting attention worldwide as a functional food. This is linked with its high protein content and its balanced amino acid profile. During the passage of quinoa proteins through the digestive tract, they undergo modifications resulting from the action of gastrointestinal enzymes, releasing peptides with potential health benefits. In the last years, the search for food-derived antioxidant and antiproliferative peptides has gained interest. The aim of this study was to evaluate the potential of quinoa proteins as source of peptides exerting these activities after their in vitro digestion simulating physiological conditions. A quinoa protein concentrate was sequentially digested with pepsin and pancreatin following the internationally harmonized in vitro protocol. Digests were collected at the end of gastric phase, and after 60 min and 120 min incubation with pancreatin. Evaluation of the antioxidant (ORAC assay) and anti-proliferative effects using colon cancer Caco-2, HT-29, and HCT-116 cell lines was performed before and after ultrafiltration using 5kDa membranes. The gastroduodenal digest at 60 min showed an ORAC value of 2.22 μmol of Trolox equivalent/mg protein, and the observed IC50 (protein concentration needed to inhibit 50% cell viability) values were ranged between 0.21 and 0.28 mg/ml in the different celllines. The analysis of the molecular weight distribution by MALDI-TOF and the amino acid profile indicated that the short size and high proportion of aromatic and hydrophobic amino acids of peptides contained in fraction lower than 5 kDa appear responsible for the …

Año de publicación:

2017

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