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Water Dynamics of Mung bean (Vigna radiata) Sprouts Treated with 6-Benzylaminopurine: Discrimination by Low-Field Nuclear Magnetic Resonance and Spectrometry

Abstract:

Mung bean sprouts are a kind of favorite healthy food across the world. As a forbidden plant hormone cytokinins, the use of 6-benzylaminopurine (6-BAP) in promoting the growth of bean sprouts is forbidden due to its safety concerns. Rapid analysis of 6-BAP treated bean sprouts is required to assess their growth changes and discriminate them from the normal sample. In this study, the water dynamics of growth for 6-BAP treated bean sprouts was monitored by the low-field nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging. A simplified cartoon model of the water dynamics was proposed to explain the LF-NMR analysis result. Principal component analysis of the NMR relaxation data clearly showed the different water distribution of 6-BAP treated bean sprouts from the control sample. The juice of 6-BAP treated bean sprouts showed significant difference in the ultraviolet absorption and fluorescence spectra. Therefore, combining the spectrometry, the LF-NMR may have potential for rapid discrimination of the 6-BAP treated bean sprouts.