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Capillary electrophoresis: Novel tool for simmondsins analysis and its application to jojoba breeding

Abstract:

Jojoba seed, a rich source of liquid wax, contains also a group of closely related glycosides: simmondsin (SM), demethylsimmondsin and didemethylsimmondsin (DDS). Four ferulates derivatives of SM and demethylsimmondsin have been identified and isolated. Growing interest and potential for application of SMs as feed additives calls for efficient and quick analytical methods both for quantitative and qualitative analyses. The HPLC method is commonly used for SMs analysis; however, the presence of stereosomers of ferulates may require more precise, faster and less expensive analytical method, especially for animal studies. In this report, it is shown that capillary electrophoresis (CE) is a powerful separation and quantitation technique that provides high resolving power, short analysis time, and low operational cost for the analysis of SM derivatives. The CE method for analysis of SMs was developed, scrutinized in interlaboratory ring test, and applied to monitoring the breeding studies. Although CE is not commonly used for analysis of plant material or agriculture products, it has shown great potential for the analysis substances of interest in biological matrices or profiling metabolites of physiologically important molecules. The exposure of simmondsin ferulates (SSFs) to light causes their isomerization, and this effect may be easily quantified by CE. The CE method is also applicable to quality control analysis for processing and purification of SMs used as animal feed additive. (C) 2000 Elsevier Science B.V.