Mostrando 10 resultados de: 67
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Biotecnologia Aplicada(12)
Electrophoresis(10)
Analytical Biochemistry(9)
Biomolecules(2)
Frontiers in Physiology(2)
Comparative Serum Analyses Identify Cytokines and Hormones Commonly Dysregulated as Well as Implicated in Promoting Osteolysis in MMP-2-Deficient Mice and Children
ArticleAbstract: Deficiency of matrix metalloproteinase 2 (MMP-2) causes a complex syndrome characterized by multicenPalabras claves:cortisol, cytokines, Matrix metalloproteinase, matrix metalloproteinase-2, multicentric osteolysis nodulosis and arthropathyAutores:Botto L.D., Eugenio Hardy, Fernandez‐Patron C., Haimour A., Karim M.A., Martignetti J.A., Sarker H., Scholl-Bürgi S.Fuentes:scopusComparison of two gel electrophoresis-based DNA micropurification methods for molecular cloning
ArticleAbstract: We recently described a method for recovering nucleic acids separated from polyacrylamide gels, whicPalabras claves:Cloning, Ethidium bromide, Gel electrophoresis, Ultraviolet-light, Zinc stainingAutores:Castellanos‐Serra L., Coizeau E., Eugenio Hardy, Hermida L., Niebla O., Pupo E., Silva R.Fuentes:scopusComplexity and solutions to the isolation problem of Gram negative lipopolysaccharides' bacteria molecular species
ReviewAbstract: Gram negative bacteria frequently synthesize a great number of lipopolysaccharides (LPS) molecular sPalabras claves:chromatography, Electrophoresis, Elution, Isolation, lipopolysaccharides, Mass spectrometry, Reverse stainingAutores:Eugenio Hardy, Pupo E.Fuentes:scopusBiopharmaceutical formulations of liposome-associated desoxyribonucleic acids
ReviewAbstract: In this paper the current development of liposome-based DNA formulations as a biopharmaceutical prodPalabras claves:delivery system, Gene therapy, LiposomeAutores:Eugenio Hardy, Pupo E.Fuentes:scopusAntigenicity and immunogenicity of a synthetic oligosaccharide-protein conjugate vaccine against Haemophilus influenzae type b
ArticleAbstract: Polysaccharide-protein conjugates as vaccines have proven to be very effective in preventing HaemophPalabras claves:Autores:Cardoso F., Carmenate T., Chang J., Cosme K., Eugenio Hardy, Fernández-Santana V., Figueroa I., Hernández I., Heynngnezz L., Izquierdo M., Mawas F., Musacchio Lasa A., Peña L., Rodriguez A., Rodriguez M.C., Roy R., Toledo-Romani M.E., Valdés Y., Verez-Bencomo V.Fuentes:scopus"Reverse-staining" of biomolecules in electrophoresis gels: Analytical and micropreparative applications
ReviewAbstract: Negative or reverse staining using imidazole and zinc salts for protein detection in electrophoresisPalabras claves:Gel electrophoresis, lipopolysaccharides, Negative staining of gels, Nucleic acids, proteins, Proteomic purification, Proteomic sample preparation, Reverse staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusA New Science Publishing System for a Budding Science Publishing Crisis
ArticleAbstract: The current science publishing system is in need of a positive transformation for the good of scientPalabras claves:Journal, METRICS, Open access, Science publishingAutores:Eugenio Hardy, Fernandez‐Patron C.Fuentes:scopusA modified variant of human Interleukin-15 as a novel antigen for active immunotherapy in rheumatoid arthritis
ReviewAbstract: Interleukin (IL)-15 is a pro-inflammatory cytokine that plays a crucial role in the pathogenesis ofPalabras claves:IL-15, Immunization, neutralizing antibodies, non-human primates, Rheumatoid ArthritisAutores:Alexey Llópiz-Arzuaga, Arrieta C.A., Besada-Pérez V., Castro-Velazco J., Eugenio Hardy, Geronimo H., Guillén-Nieto G.E., Martínez K., Martínez-Castillo R., Morera-Díaz Y., Moro A., Perea-Rodríguez S.E., Puente P., Ramos Y., Rodríguez-Álvarez Y., Santos-Savio A., Silva R., Vázquez M.Fuentes:scopusA procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
ArticleAbstract: We developed a technique that allows rapid protein elution from polyacrylamide gel bands at room temPalabras claves:Passive elution, Protein detection, Protein micropurification, Sodium dodecyl, Sulfate-polyacrylamide gel electrophoresis, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V.Fuentes:scopusA sandwich-type enzyme-linked immunosorbent assay for the analysis of recombinant human interferon α-2b
ArticleAbstract: The ability of a previously developed sandwich-type enzyme-linked immunosorbent assay (ELISA) for diPalabras claves:Elisa, ESCHERICHIA coli, folding, IFN-alpha, monoclonal antibodiesAutores:Espino Y., Eugenio Hardy, Franco A., Furrazola G., Santana-Milián H.Fuentes:scopus