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Comparison of two gel electrophoresis-based DNA micropurification methods for molecular cloning
ArticleAbstract: We recently described a method for recovering nucleic acids separated from polyacrylamide gels, whicPalabras claves:Cloning, Ethidium bromide, Gel electrophoresis, Ultraviolet-light, Zinc stainingAutores:Castellanos‐Serra L., Coizeau E., Eugenio Hardy, Hermida L., Niebla O., Pupo E., Silva R.Fuentes:scopusComplexity and solutions to the isolation problem of Gram negative lipopolysaccharides' bacteria molecular species
ReviewAbstract: Gram negative bacteria frequently synthesize a great number of lipopolysaccharides (LPS) molecular sPalabras claves:chromatography, Electrophoresis, Elution, Isolation, lipopolysaccharides, Mass spectrometry, Reverse stainingAutores:Eugenio Hardy, Pupo E.Fuentes:scopus"Reverse-staining" of biomolecules in electrophoresis gels: Analytical and micropreparative applications
ReviewAbstract: Negative or reverse staining using imidazole and zinc salts for protein detection in electrophoresisPalabras claves:Gel electrophoresis, lipopolysaccharides, Negative staining of gels, Nucleic acids, proteins, Proteomic purification, Proteomic sample preparation, Reverse staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusA procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
ArticleAbstract: We developed a technique that allows rapid protein elution from polyacrylamide gel bands at room temPalabras claves:Passive elution, Protein detection, Protein micropurification, Sodium dodecyl, Sulfate-polyacrylamide gel electrophoresis, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V.Fuentes:scopusA sandwich-type enzyme-linked immunosorbent assay for the analysis of recombinant human interferon α-2b
ArticleAbstract: The ability of a previously developed sandwich-type enzyme-linked immunosorbent assay (ELISA) for diPalabras claves:Elisa, ESCHERICHIA coli, folding, IFN-alpha, monoclonal antibodiesAutores:Espino Y., Eugenio Hardy, Franco A., Furrazola G., Santana-Milián H.Fuentes:scopusDetection of PEGylated proteins in polyacrylamide gels by reverse staining with zinc and imidazole salts
ArticleAbstract: The reverse staining, with imidazole-SDS-zinc, of PEG-linked proteins separated by SDS-PAGE was studPalabras claves:Gel electrophoresis, PEGylated interferon, Protein staining, Reverse staining, Zinc stainingAutores:Báez R., Eugenio Hardy, Ramon J., Ruiz A., Saez V., Tejeda Y.Fuentes:scopusDetection of biomolecules in electrophoresis gels with salts of imidazole and zinc II: A decade of research
ReviewAbstract: The proven ability of gel electrophoresis to simultaneously resolve, in a single experiment, many coPalabras claves:Gel electrophoresis detection, Imidazole zinc staining, lipopolysaccharides, Nucleic acids, proteins, Reverse staining, ReviewAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusExpression and folding of an interleukin-2-proinsulin fusion protein and its conversion into insulin by a single step enzymatic removal of the C-peptide and the N-terminal fused sequence
ArticleAbstract: We report the expression in E. coli of a proinsulin fusion protein carrying a modified interleukin-2Palabras claves:Enzymatic processing, Proinsulin folding, Recombinant insulinAutores:Besada-Pérez V., Castellanos‐Serra L., Eugenio Hardy, Falcón-Cama V., Fernandez C., Gonzalez M., Herrera L., Perez G., Santiago Nelson Vispo, Santos-Savio A., Silva A., Ubieta R.Fuentes:scopusElution of lipopolysaccharides from polyacrylamide gels
ArticleAbstract: A simple procedure for elution in water of bacterial lipopolysaccharides (LPS) from sodium dodecyl sPalabras claves:Elution, Gel electrophoresis, LAL, Silver staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio Hardy, Guerra M., Pupo E., Santana-Milián H.Fuentes:scopusElution of unmodified oligodeoxynucleotides from zinc-Imidazole negatively stained polyacrylamide gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Coizeau E., Eugenio Hardy, Pupo E., Silva J.A., Silva R.Fuentes:scopus