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Complexity and solutions to the isolation problem of Gram negative lipopolysaccharides' bacteria molecular species
ReviewAbstract: Gram negative bacteria frequently synthesize a great number of lipopolysaccharides (LPS) molecular sPalabras claves:chromatography, Electrophoresis, Elution, Isolation, lipopolysaccharides, Mass spectrometry, Reverse stainingAutores:Eugenio Hardy, Pupo E.Fuentes:scopusBiopharmaceutical formulations of liposome-associated desoxyribonucleic acids
ReviewAbstract: In this paper the current development of liposome-based DNA formulations as a biopharmaceutical prodPalabras claves:delivery system, Gene therapy, LiposomeAutores:Eugenio Hardy, Pupo E.Fuentes:scopus"Reverse-staining" of biomolecules in electrophoresis gels: Analytical and micropreparative applications
ReviewAbstract: Negative or reverse staining using imidazole and zinc salts for protein detection in electrophoresisPalabras claves:Gel electrophoresis, lipopolysaccharides, Negative staining of gels, Nucleic acids, proteins, Proteomic purification, Proteomic sample preparation, Reverse staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusA procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
ArticleAbstract: We developed a technique that allows rapid protein elution from polyacrylamide gel bands at room temPalabras claves:Passive elution, Protein detection, Protein micropurification, Sodium dodecyl, Sulfate-polyacrylamide gel electrophoresis, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V.Fuentes:scopusA synthetic conjugate polysaccharide vaccine against haemophilus influenzae type b
ArticleAbstract: Glycoconjugate vaccines provide effective prophylaxis against bacterial infections. To date, howeverPalabras claves:Autores:Aguilar A., Baly A., Campa C., Cardoso F., Carmenates A., Cosme K., Costa L., Deler M.L., Diaz M., Eugenio Hardy, Fernández-Santana V., Garcia E., Hernández I., Herrera L., Heynngnezz L., Izquierdo M., Medina E., Montane M., Muzachio A., Ramos A., Rodriguez A., Rodriguez M.C., Roy R., Sánchez R., Sosa I., Toledo-Romani M.E., Toraño G., Valdés Y., Verez-Bencomo V., Villar A.Fuentes:scopusActive immunization with human interleukin-15 induces neutralizing antibodies in non-human primates
ArticleAbstract: Background: Interleukin-15 is an immunostimulatory cytokine overexpressed in several autoimmune andPalabras claves:Alum, CTLL-2 cells, Cytokine, IL-15, Immunization, Neutralizing Abs, non-human primatesAutores:Besada-Pérez V., Castro-Velazco J., Chico-Capote A., Eugenio Hardy, Gerónimo-Pérez H., Martínez-Castillo R., Martínez-Cordovez K., Morera-Díaz Y., Puente-Pérez P., Rodríguez-Álvarez Y., Santos-Savio A.Fuentes:scopusGlucocorticoids Bind to SARS-CoV-2 S1 at Multiple Sites Causing Cooperative Inhibition of SARS-CoV-2 S1 Interaction With ACE2
ArticleAbstract: Dexamethasone may reduce mortality in COVID-19 patients. Whether dexamethasone or endogenous glucocoPalabras claves:ACE2 (angiotensin converting enzyme 2), CORONAVIRUS, covid, glucocorticoids, innate immunity, SARS-COV-2Autores:Elahi S., Eugenio Hardy, Fernandez‐Patron C., Glover J.N.M., Panigrahi R., Sarker H.Fuentes:scopusExpression of il-2/proinsulin fusion protein: Folding of the fusion protein and conversion into insulin by a one step removal of c-peptide and leader sequence
ArticleAbstract:Palabras claves:Autores:Besada-Pérez V., Castellanos L.R., Estrada R., Eugenio Hardy, Fernandez C., Gonzalez M., Herrera L., Jimenez V., Perez G., Santiago Nelson Vispo, Silva A., Ubieta R.Fuentes:scopusFast passive elution of DNA from zinc-imidazole negatively stained polyacrylamide gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Sánchez J.C.Fuentes:scopusEnhanced ELISA sensitivity using TCA for efficient coating of biologically active lipopolysaccharides or lipid A to the solid phase
ArticleAbstract: A new simple, reproducible and sensitive ELISA that uses trichloroacetic acid (TCA) for the coatingPalabras claves:Coating efficiency, Elisa, Lipid A, LIPOPOLYSACCHARIDE, Trichloroacetic acid ([76-03-9])Autores:Eugenio Hardy, Llano M., Ohlin M.Fuentes:scopus