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"Reverse-staining" of biomolecules in electrophoresis gels: Analytical and micropreparative applications
ReviewAbstract: Negative or reverse staining using imidazole and zinc salts for protein detection in electrophoresisPalabras claves:Gel electrophoresis, lipopolysaccharides, Negative staining of gels, Nucleic acids, proteins, Proteomic purification, Proteomic sample preparation, Reverse staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusA New Science Publishing System for a Budding Science Publishing Crisis
ArticleAbstract: The current science publishing system is in need of a positive transformation for the good of scientPalabras claves:Journal, metrics, Open access, Science publishingAutores:Eugenio Hardy, Fernandez‐Patron C.Fuentes:scopusA modified variant of human Interleukin-15 as a novel antigen for active immunotherapy in rheumatoid arthritis
ReviewAbstract: Interleukin (IL)-15 is a pro-inflammatory cytokine that plays a crucial role in the pathogenesis ofPalabras claves:IL-15, Immunization, neutralizing antibodies, non-human primates, Rheumatoid ArthritisAutores:Alexey Llópiz-Arzuaga, Arrieta C.A., Besada V., Besada-Pérez V., Castro-Velazco J., Eugenio Hardy, Geronimo H., Guillén-Nieto G.E., Klaudia Martínez, Martínez K., Martínez-Castillo R., Morera-Díaz Y., Moro A., Perea-Rodríguez S.E., Puente P., Rafael Martínez, Ramos Y., Rodríguez-Álvarez Y., Santos-Savio A., Silva R., Vázquez M.Fuentes:scopusA procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
ArticleAbstract: We developed a technique that allows rapid protein elution from polyacrylamide gel bands at room temPalabras claves:Passive elution, Protein detection, Protein micropurification, Sodium dodecyl, Sulfate-polyacrylamide gel electrophoresis, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V.Fuentes:scopusA sandwich-type enzyme-linked immunosorbent assay for the analysis of recombinant human interferon α-2b
ArticleAbstract: The ability of a previously developed sandwich-type enzyme-linked immunosorbent assay (ELISA) for diPalabras claves:Elisa, ESCHERICHIA coli, folding, IFN-alpha, monoclonal antibodiesAutores:Espino Y., Eugenio Hardy, Franco A., Furrazola G., Santana-Milián H.Fuentes:scopusA synthetic conjugate polysaccharide vaccine against haemophilus influenzae type b
ArticleAbstract: Glycoconjugate vaccines provide effective prophylaxis against bacterial infections. To date, howeverPalabras claves:Autores:Aguilar A., Baly A., Campa C., Cardoso F., Carmenates A., Cosme K., Costa L., Deler M.L., Diaz M., Ernesto Garcia, Eugenio Hardy, Fernández-Santana V., Garcia E., Hernández I., Herrera L., Heynngnezz L., Izquierdo M., Manuel Diaz, Maria C. Rodríguez, Medina E., Montane M., Muzachio A., Ramos A., Raydel Martínez, Rodriguez A., Rodriguez M.C., Roy R., Sánchez R., Sosa I., Toledo-Romani M.E., Toraño G., Valdés Y., Verez-Bencomo V., Villar A.Fuentes:scopusTopical disposition of two strengths of a <sup>125</sup>I-rhEGF jelly in rat skin wounds
ArticleAbstract: Growth factors have proved to be an effective therapeutic strategy. However, some controversies havePalabras claves:125 I-rhEGF, Jelly, Non-linearity, Strength, Topical dispositionAutores:Aguilera A., Alfonso-Ortíz S., Alvarez D., Bécquer M.d.l.A., Duconge J., Estrada L., Eugenio Hardy, Fernández-Sánchez E.M., García-Pulpeiro O., Prats P.A., Rojas I., Valenzuela-Silva C.M.Fuentes:scopusSome factors affecting the stability of interferon alpha 2b in solution
ArticleAbstract: In this paper we evaluated the influence of the protein concentration and a formulation vehicle on tPalabras claves:Albumin-free formulation, INTERFERÓN, Protein concentration, stabilityAutores:Aldana R., Aroche K., Báez R., Eugenio Hardy, Reyes N., Ruiz L.Fuentes:scopusStability of Ala 125 recombinant human interleukin-2 in solution
ArticleAbstract: Herein, we describe the preformulation study of Ala 125- recombinant human interleukin-2 (rhIL-2A<suPalabras claves:Autores:Aroche K., Brito O., Eugenio Hardy, Geronimo H., Reyes N., Ruiz L.Fuentes:scopusApolipoprotein-A1 transports and regulates MMP2 in the blood
ArticleAbstract: Synthesized in the liver and intestines, apolipoprotein A1 (APOA1) transports cholesterol in high dePalabras claves:Autores:Elena Anderson, Eugenio Hardy, Fernandez‐Patron C., J. N.Mark Glover, Ken Reyes, López-Campistrous A.E., Rashmi Panigrahi, Samuel Hernandez-Anzaldo, Sarker H., Todd McMullen, Vidhya Krishnan, Xi Long ZhengFuentes:scopus