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"Reverse-staining" of biomolecules in electrophoresis gels: Analytical and micropreparative applications
ReviewAbstract: Negative or reverse staining using imidazole and zinc salts for protein detection in electrophoresisPalabras claves:Gel electrophoresis, lipopolysaccharides, Negative staining of gels, Nucleic acids, proteins, Proteomic purification, Proteomic sample preparation, Reverse staining, Zinc stainingAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusA New Science Publishing System for a Budding Science Publishing Crisis
ArticleAbstract: The current science publishing system is in need of a positive transformation for the good of scientPalabras claves:Journal, METRICS, Open access, Science publishingAutores:Eugenio Hardy, Fernandez‐Patron C.Fuentes:scopusA modified variant of human Interleukin-15 as a novel antigen for active immunotherapy in rheumatoid arthritis
ReviewAbstract: Interleukin (IL)-15 is a pro-inflammatory cytokine that plays a crucial role in the pathogenesis ofPalabras claves:IL-15, Immunization, neutralizing antibodies, non-human primates, Rheumatoid ArthritisAutores:Alexey Llópiz-Arzuaga, Arrieta C.A., Besada-Pérez V., Castro-Velazco J., Eugenio Hardy, Geronimo H., Guillén-Nieto G.E., Martínez K., Martínez-Castillo R., Morera-Díaz Y., Moro A., Perea-Rodríguez S.E., Puente P., Ramos Y., Rodríguez-Álvarez Y., Santos-Savio A., Silva R., Vázquez M.Fuentes:scopusA procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
ArticleAbstract: We developed a technique that allows rapid protein elution from polyacrylamide gel bands at room temPalabras claves:Passive elution, Protein detection, Protein micropurification, Sodium dodecyl, Sulfate-polyacrylamide gel electrophoresis, Trace enrichmentAutores:Castellanos‐Serra L., Eugenio Hardy, Fernandez‐Patron C., Huerta V.Fuentes:scopusA sandwich-type enzyme-linked immunosorbent assay for the analysis of recombinant human interferon α-2b
ArticleAbstract: The ability of a previously developed sandwich-type enzyme-linked immunosorbent assay (ELISA) for diPalabras claves:Elisa, ESCHERICHIA coli, folding, IFN-alpha, monoclonal antibodiesAutores:Espino Y., Eugenio Hardy, Franco A., Furrazola G., Santana-Milián H.Fuentes:scopusA synthetic conjugate polysaccharide vaccine against haemophilus influenzae type b
ArticleAbstract: Glycoconjugate vaccines provide effective prophylaxis against bacterial infections. To date, howeverPalabras claves:Autores:Aguilar A., Baly A., Campa C., Cardoso F., Carmenates A., Cosme K., Costa L., Deler M.L., Diaz M., Eugenio Hardy, Fernández-Santana V., Garcia E., Hernández I., Herrera L., Heynngnezz L., Izquierdo M., Medina E., Montane M., Muzachio A., Ramos A., Rodriguez A., Rodriguez M.C., Roy R., Sánchez R., Sosa I., Toledo-Romani M.E., Toraño G., Valdés Y., Verez-Bencomo V., Villar A.Fuentes:scopusComparative Serum Analyses Identify Cytokines and Hormones Commonly Dysregulated as Well as Implicated in Promoting Osteolysis in MMP-2-Deficient Mice and Children
ArticleAbstract: Deficiency of matrix metalloproteinase 2 (MMP-2) causes a complex syndrome characterized by multicenPalabras claves:cortisol, cytokines, Matrix metalloproteinase, matrix metalloproteinase-2, multicentric osteolysis nodulosis and arthropathyAutores:Botto L.D., Eugenio Hardy, Fernandez‐Patron C., Haimour A., Karim M.A., Martignetti J.A., Sarker H., Scholl-Bürgi S.Fuentes:scopusComparison of two gel electrophoresis-based DNA micropurification methods for molecular cloning
ArticleAbstract: We recently described a method for recovering nucleic acids separated from polyacrylamide gels, whicPalabras claves:Cloning, Ethidium bromide, Gel electrophoresis, Ultraviolet-light, Zinc stainingAutores:Castellanos‐Serra L., Coizeau E., Eugenio Hardy, Hermida L., Niebla O., Pupo E., Silva R.Fuentes:scopusComplexity and solutions to the isolation problem of Gram negative lipopolysaccharides' bacteria molecular species
ReviewAbstract: Gram negative bacteria frequently synthesize a great number of lipopolysaccharides (LPS) molecular sPalabras claves:chromatography, Electrophoresis, Elution, Isolation, lipopolysaccharides, Mass spectrometry, Reverse stainingAutores:Eugenio Hardy, Pupo E.Fuentes:scopusBiopharmaceutical formulations of liposome-associated desoxyribonucleic acids
ReviewAbstract: In this paper the current development of liposome-based DNA formulations as a biopharmaceutical prodPalabras claves:delivery system, Gene therapy, LiposomeAutores:Eugenio Hardy, Pupo E.Fuentes:scopus