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A modified variant of human Interleukin-15 as a novel antigen for active immunotherapy in rheumatoid arthritis
ReviewAbstract: Interleukin (IL)-15 is a pro-inflammatory cytokine that plays a crucial role in the pathogenesis ofPalabras claves:IL-15, Immunization, neutralizing antibodies, non-human primates, Rheumatoid ArthritisAutores:Alexey Llópiz-Arzuaga, Arrieta C.A., Besada-Pérez V., Castro-Velazco J., Eugenio Hardy, Geronimo H., Guillén-Nieto G.E., Martínez K., Martínez-Castillo R., Morera-Díaz Y., Moro A., Perea-Rodríguez S.E., Puente P., Ramos Y., Rodríguez-Álvarez Y., Santos-Savio A., Silva R., Vázquez M.Fuentes:scopusComparison of two gel electrophoresis-based DNA micropurification methods for molecular cloning
ArticleAbstract: We recently described a method for recovering nucleic acids separated from polyacrylamide gels, whicPalabras claves:Cloning, Ethidium bromide, Gel electrophoresis, Ultraviolet-light, Zinc stainingAutores:Castellanos‐Serra L., Coizeau E., Eugenio Hardy, Hermida L., Niebla O., Pupo E., Silva R.Fuentes:scopusComplexity and solutions to the isolation problem of Gram negative lipopolysaccharides' bacteria molecular species
ReviewAbstract: Gram negative bacteria frequently synthesize a great number of lipopolysaccharides (LPS) molecular sPalabras claves:chromatography, Electrophoresis, Elution, Isolation, lipopolysaccharides, Mass spectrometry, Reverse stainingAutores:Eugenio Hardy, Pupo E.Fuentes:scopusBiopharmaceutical formulations of liposome-associated desoxyribonucleic acids
ReviewAbstract: In this paper the current development of liposome-based DNA formulations as a biopharmaceutical prodPalabras claves:delivery system, Gene therapy, LiposomeAutores:Eugenio Hardy, Pupo E.Fuentes:scopusDesign and validation of a diffusion cell for in vitro biomolecule release studies
ArticleAbstract: In this work, a new cell for the in vitro release of biomolecules was designed and built. The cell,Palabras claves:Diffusion cell, EGF, Epidermal growth factor, In vitro release, ValidationAutores:Aguilera A., Estevez T., Eugenio Hardy, Saez A.Fuentes:scopusFeasibility, via a new interface, of mass spectrometry of lipopolysaccharides separated in polyacrylamide gels
ArticleAbstract: For the first time, it was demonstrated the feasibility of analyzing by mass spectrometry (MS) Gram-Palabras claves:Autores:Eugenio Hardy, Pupo E.Fuentes:scopusNew tools in proteomics: A high sequence coverage by protein digestion in polyacrylamide IEF-CA, IEF-IPG and SDS gels
ArticleAbstract:Palabras claves:Autores:Castellanos‐Serra L., Eugenio Hardy, Huerta V., Javier González L.Fuentes:scopusScale-up of the polyacrylamide gel electrophoresis-reverse staining-extrusion-passive elution technique for the straightforward recovery of milligrams of recombinant proteins
ArticleAbstract: Forcing excised gel fragments through a metal sieve of 32 μm average mesh size contained in the bottPalabras claves:Elution, Gel electrophoresis, Pertactin, Protein, purification, Recombinant proteinAutores:Del Carmen Abrahantes M., Eugenio Hardy, Pentón G., Quintana D.Fuentes:scopusSelective precipitation of zinc imidazolate: Application to the detection of biomolecules (proteins, nucleic acids, and bacterial glycolipids) separated in polyacrylamide gels
ArticleAbstract: At the start of the past decade a group of scientists from the Center for Genetic Engineering and BiPalabras claves:Electrophoresis, lipopolysaccharides, Negative staining, Nucleic acids, Polyacrylamide, proteinsAutores:Castellanos‐Serra L., Eugenio HardyFuentes:scopusOn the isolation of immunostimulatory active acemannan from Aloe barbadensis
ArticleAbstract: Acemannan from Aloe barbadensis was obtained with four processes: 1) size exclusion chromatography (Palabras claves:Acemannan, Aloe Vera, Cetyltrimethylammonium bromide, HBsAg, purificationAutores:Aguilar J., Alonso M., Cremata J.A., Eugenio Hardy, Gerwig G., Kamerling H., López M., Mayo O., Prieto E., Támbara Y.Fuentes:scopus